熄风胶囊对氯化锂-匹罗卡品癫痫大鼠海马损伤影响的研究

Effect of Xi Feng capsule on hippocampal lesion in rats with epilepsy induced by lithium-pilocarpine

关键词:  癫痫  熄风胶囊  海马  氯化锂-匹罗卡品  反复自发性发作  苔藓纤维出芽

Key words:  epilepsy  Xi Feng capsule  hippocampus  lithium-pilocarpine  spontaneous recurrent seizures  mossy fiber sprouting

摘要:

[目的] 探讨熄风胶囊对氯化锂-匹罗卡品癫痫大鼠海马损伤的影响.[方法] 应用氯化锂-匹罗卡品复制难治性癫痫大鼠模型.观察实验大鼠的反复自发性发作(SRS)情况、海马形态学改变和苔藓纤维出芽(MFS).[结果] 1)治疗组癫痫大鼠SRS的发作次数均明显低于模型组(P < 0.01).2)光、电镜结果显示：各治疗组中联合治疗组海马结构损伤最轻.3)Timm染色结果显示：联合治疗组对海马CA3区及齿状回MFS有较强的干预作用,优于单药治疗组.这种干预作用的强弱与熄风胶囊的剂量呈现一定正相关关系.[结论] 熄风胶囊单药及与卡马西平(CBZ)联合用药能够有效的控制氯化锂-匹罗卡品癫痫大鼠的SRS,降低海马神经元损伤的程度,抑制苔藓纤维异常出芽.

Abstract:

[Objective] To study the effect of Xi Feng capsule on hippocampal lesion in rats with epilepsy induced by Lithium-pilocarpine. [Methods] Lithium-pilocarpine was used to duplicate the epilepsy in rats. Then the spontaneous recurrent seizures(SRS) of rats was observed and recorded by the vedio control system. The conventional histopathological method and transmission electron microscope were utilized to observe the morphological changes in hippocampus. Timm histochemical technique was adopted to study mossy fiber sprouting(MFS). [Results] 1)The recurrent time of SRP in treatment group was much less than model group (P < 0.01). 2)Conventional histopathological method and transmission electron microscope found that, in all therapy groups, the damage of neuron in hippocampus was ameliorated in Xi Feng high-dose + Carbamazepine(CBZ)1/2 dose group. 3)The result of Timm stain method suggested that the hippocampal CA3 subfield in Xi Feng high-dose + CBZ 1/2 dose group was less than that of Xi Feng single group. This effect of Xi Feng capsule could be increased as its dose increased. [Conclusion] Xi Feng capsule only and combined with CBZ can control the SRS of rat with epilepsy induced by lithium-pilocarpine, and protect hippocampal neurons from more serious lesson and inhibit mossy hippocampal MFS.