空心莲子草有效部位提取物抗甲型H3N2流感病毒作用

Antiviral activity of Alternanthera Philoxeroides effective fractions extracts against virus A H3N2

关键词:  空心莲子草  MDCK细胞  甲型H3N2流感病毒  抗病毒作用

Key words:  Alternanthera philoxeroidis  MDCK cells  influenza A Virus H3N2  antiviral effect

摘要:

[目的] 以空心莲子草有效部位提取物为研究对象, 进一步验证其抗甲型H3N2流感病毒的生物活性, 为其开发应用提供依据。[方法] 以利巴韦林注射液作为空心莲子草有效部位提取物抗病毒实验的阳性对照药物, 观察甲型H3N2流感病毒感染幼犬肾(MDCK)细胞后引起的细胞病变效应(CPE), 通过四甲基偶氮唑蓝(MTT)法检测细胞活性, 采用治疗指数(TI)作为药物抗病毒效果的评价指标, 评价空心莲子草有效部位提取物抗甲型H3N2流感病毒的生物活性。 [结果] 空心莲子草有效部位提取物对流感病毒有明显抗病毒生物合成作用, 其TI为(2.43±0.01), 但其阻止流感病毒吸附与直接杀伤流感病毒的作用不明显。[结论] 空心莲子草有效部位提取物有较强的抗流感病毒作用, 其作用方式为抗病毒生物合成, 且呈剂量依赖性。

Abstract:

[Objective] Using Alternanthera Philoxeroides effective fractions extracts as a research object, to further validate its biological activity in vitro anti-influenza A H3N2 influenza virus, in order to provide a basis for its development and application. [Methods] Ribavirin injection was used as a positive control drug. By observing the cytopathic effect (CPE) caused by the influenza A H3N2 influenza virus infection in MDCK cells, using MTT method to check the cell activity, and using therapeutic index (TI) as an evaluation of antiviral effect in vitro to evaluate the antiviral activity of the drugs. To evaluate the biological activity against influenza A H3N2 influenza virus of Alternanthera Philoxeroides effective fractions extracts. [Results] Alternanthera Philoxeroides effective fractions of extracts had significant antiviral biosynthesis of influenza virus. Its therapeutic index (TI) were (2.43±0.01). But its preventing the influenza virus adsorption and direct anti-influenza virus was not obvious. [Conclusion] Alternanthera Philoxeroides effective fractions extracts have certain anti-influenza virus, and its form is antiviral biosynthesis of influenza virus, and has a dose-dependent.