针刺手法对家兔穴区组织损伤的形态学观察

Morphological observation of different acupuncture needle manipulation methods on morphology of tissue damage of acupoint point in rabbits

关键词:  针刺手法  提插  捻转  损伤

Key words:  manipulation of acupuncture  lifting-thrusting  twirling  damage

摘要:

[目的] 观察针刺治疗中3种基本操作手法(提插、捻转、提插捻转复合手法)对家兔穴区组织造成损伤形态计量学影响。[方法] 36只家兔随机分入提插组,捻转组,提插捻转组和对照组,每组9只。对家兔足三里穴分别施3种操作手法,每日操作1次,行针时间1 min,共10次。计数穴区炎细胞数量;观察组织苏木精-伊红素(HE)染色和Masson染色后组织形态学变化。[结果] 3组针刺组炎细胞数量差异无统计学意义,病理组织形态学结果显示提插组神经损伤最轻,捻转组和提插捻转组出现的神经损伤较重。[结论] 在不影响针感的前提下,在有神经干分布的穴位上针刺操作时采用单一提插法,可以减轻对神经组织的损伤。

Abstract:

[Objective] To explore the tissue damage of acupoint in rabbits by three basic manipulations of acupuncture techniques (lifting-thrusting, twirling and mixed lifting-thrusting and twirling) in acupuncture treatment. [Methods] Thirty-six rabbits were randomly divided into the lifting-thrusting (LT) group, the twirling (TW) group, mixed lifting-thrusting and twirling (M) group and normal control group, with 9 rats in each group. Needling the Zusanli point (ST36) was performed in LT group,TW group and M group with three manipulations. 10 days later the animals were euthanized. The pathologic examination of neurons and muscle tissue and amount of inflammatory cells were observed. [Results] The three needling groups were not different in the grade of inflammatory cells infiltration. The results indicated that thd nerve injury of the morphology of the pathological tissues in the lifting-thrusting group. The nerver injury in lifting-thrusting group and twirling group was more severe. [Conclusion] On the premise of no difference in acupuncture stimulation using lifting-thrusting manipulation can reduce the damage of nerve tissue in acupoint.