益气活血方调节心衰大鼠NCX和SERCA活性平衡的机制研究

Studies of quality standard about Fufang Dangshen capsules

关键词:  益气活血方  心衰大鼠  胞膜上的Na⁺/Ca²⁺交替蛋白(NCX)  肌浆网上的钙浆(SERCA)

Key words:  Fufang Dangshen capsules  quality control  TLC  UV-spectrophotometry

摘要:

[目的] 建立复方党参胶囊的质量控制方法。[方法] 采用薄层色谱法（TLC）对制剂中党参、白术、香附、黑芝麻进行定性鉴别；采用紫外分光光度法测定制剂中总多糖含量。[结果] TLC法可专属性地鉴别党参、白术、香附和黑芝麻，且特征斑点圆整清晰，阴性对照无干扰。含量测定结果显示，总多糖在60.96~304.8 μg范围线性关系良好，r=0.999 5（n=8），平均加样回收率为99.79%（RSD 1.98%，n=6）.[结论] 所建立的方法简便可行、专属性强、重复性好，可有效控制复方党参胶囊的质量。

Abstract:

[Objective] To study and establish the quality control methods for Fufang Dangshen capsules.[Methods] The method of TLC was used to identify Radix Codonopsis,Rhizoma Atractylodis Macrocephalae,Rhizoma Cyperi and Sesami Semen Nigrun. The content of polysaccharose was determined by ultraviolet spectrophotometric method. [Results] Four herbal drugs were well identified by TLC,the spots were clear,round and without interference. Quantitative analysis of ultraviolet spectrophotometric method showed that the linear range of determination polysaccharose was 60.96~304.8 μg,r=0.999 5(n=8). The average recovery of determination was 99.79% and the RSD was 1.98% (n=6). [Conclusion] This method is simple,reliable and high specific with good repeatability,and can be used for the quality control of Fufang Dangshen capsules.