| 摘要: |
| [目的] 探讨二仙汤对顺铂(CDDP)损伤后小鼠卵巢功能的调节作用。[方法] 采用化疗损伤性小鼠卵巢功能早衰模型,给予二仙汤周期灌胃,检测血清生殖激素水平、卵巢排卵计数、卵巢组织中丙二醛(MDA)的含量及总抗氧化能力(T-AOC),观察卵巢形态学变化。[结果] 与卵巢早衰模型组比较,二仙汤给药组血清卵泡刺激素(FSH)和黄体生成素(LH)降低,而雌二醇(E2)和孕酮(P)均升高;卵巢组织MDA降低,T-AOC升高,卵泡计数增加,卵泡闭锁减少,细胞间质增生减低,卵巢纤维化状况改善。[结论] 二仙汤可通过抑制氧化损伤作用,降低细胞外基质的形成,加速细胞外基质降解,从而改善卵泡和颗粒细胞功能状态,提升卵巢功能。 |
| 关键词: 二仙汤 生殖激素 卵巢功能 |
| DOI：10.11656/j.issn.1672-1519.2013.05.15 |
| 分类号: |
| 基金项目:国家自然科学基金项目(81173443);辽宁省科学技术计划(2009225010-47);辽宁省“百千万人才工程”培养经费(2009921040);辽宁省教育厅优秀人才扶持计划(高校杰出青年学者成长计划)项目(LJQ2011087) |
|
| Effect of Er Xian decoction on ovary function after injury of cisplatin in mice |
|
AI Hao1,2, ZHANG Hai-ying3, ZHANG Yu-li4, XUE Xiao-ou4
|
|
1.Department of Obstetrics and gynecology, The Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, China;2.Key Laboratory of Follicular Development and Reproduction Regulation of Liaoning Province, Jinzhou 121000, China;3.Liao ning Medical College, Jinzhou 121000, China;4.Dongzhimen Hospital Affiliated to Beijing University of TCM, Beijing 100700, China
|
| Abstract: |
| [Objective] To investigate the regulatory effect of Er Xian decoction on restoring ovary function in premature ovarian failure (POF) in mice injured by cisplatin (CDDP). [Methods] Female SD mice were given with cisplantin daily to set up the model of POF. The changes of genital hormones, ovulation, MDA, T-AOC and ovary morphology were observed in mice after intragastric administration of Er Xian decoction. [Results] Compared with model group, in the Er Xian decoction group FSH and LH decreased, E2 and P increased, MDA decreased and T-AOC increased in ovary tissue, less follicular atresia and intercellular substance presented, the fibrous degeneration of ovaries were ameliorated. [Conclusion] Er Xian decoction can restrain oxidative injury, reduce the formation of extracellular matrix and promote its degradation, thus can restore the function of follicle and granulocyte and improve ovary function. |
| Key words: Er Xian decoction reproduction hormone ovary function |
