仙蓟化斑胶囊对肥大细胞脱颗粒及组胺释放影响的研究

Study the effect of Xianji Huaban capsules on mast cell degranulation and histamine release

关键词:  仙蓟化斑胶囊  被动皮肤过敏反应  肥大细胞  脱颗粒  组胺

Key words:  Xianji Huaban capsules  PCA reaction  mast cell  degranulation  histamine

摘要:

[目的] 研究仙蓟化斑胶囊抗变态反应的作用机制, 为后期实验及其临床治疗过敏性紫癜提供依据。[方法] 制备致敏的大鼠及抗卵蛋白血清, 给予不同浓度药物, 采用大鼠被动皮肤过敏反应(PCA)实验, 测定大鼠皮肤蓝斑直径, 同时采用大鼠颅骨骨膜肥大细胞脱颗粒实验、大鼠肥大细胞释放组胺实验, 测定脱颗粒百分率和组胺释放水平。[结果] 仙蓟化斑胶囊可使蓝斑直径变小、可降低肥大细胞脱颗粒百分率、降低其组胺释放, 并呈明显的剂量依赖关系。[结论] 仙蓟化斑胶囊有抗变态反应的作用, 明显抑制PCA、肥大细胞脱颗粒及组胺的释放。

Abstract:

[Objective] To research the mechanism of Xianji Huaban capsules against hypersensitivity reaction, providing the foundation for later experiment and clinical treatment of allergic purpura. [Methods] By preparing the sensitization of rats and anti-egg protein in serum and giving different concentrations, we measured the skin blue spot diameter through the experimental of passive cutaneous anaphylaxis (PCA); then, we measured the degranulation percentage and the level of histamine release through the skull periosteum mast cells degranulation test and the mast cells release histamine test in rats. [Results] Xianji Huaban capsules could make the skin blue spot diameter smaller, decrease mast cells degranulation percentage, and reduce the release of histamine in a concentration dependent manner. [Conclusion] Xianji Huaban capsules have the effect against hypersensitivity reaction by inhibiting PCA, mast cells degranulation and histamine releasing.