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| 慢频率下不同针刺时间影响内关穴干预MCAO大鼠效应的实验研究 |
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杨沙1,2,3, 樊小农1,2,4, 罗丁5, 张亚男5, 魏媛媛5, 张海涛5, 陈建飞5, 贺妮娜5, 常晓波5, 王舒1,2,3, 孟智宏1, 石学敏1
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1.天津中医药大学第一附属医院针灸研究所, 天津 300193;2.国家中医药管理局脑病针刺疗法重点研究室, 天津 300193;3.天津市针灸学重点实验室, 天津 300193;4.国家中医药管理局量效关系三级实验室, 天津 300193;5.天津中医药大学, 天津 300193
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| 摘要: |
| [目的] 探讨慢频率下,不同针刺时间参数对内关穴经穴特异性的影响。[方法] 参照Zea-Longa线拴法复制大脑中动脉缺血模型(MCAO),随机分为基础组即正常组、假手术组、模型组、非针刺组以及内关短时间组(5秒)、中时间组(60秒)和长时间组(180秒)共7组,内关穴3组针刺参数是固定1次/秒频率分别应用5、60、180秒的针刺时间,并每12 h针刺1次持续6次。观察效应指标即动物神经功能情况、缺血局部微循环血流量、脑组织梗死率和机制指标包括微循环血管输入支、输出支管径,以及脑组织苏木-伊红HE染色的细胞形态学指标。模型组在造模成功即刻,后4组在造模后72 h观察指标。[结果] 内关3个组在效应指标的改善上优于模型组、非针刺组(P<0.05),机制指标中,内关皮质、海马、纹状体的微血管数、正常神经细胞数(P<0.05)增加,对各项指标的改善,以中长时间较优。[结论] 针刺内关穴可获得较好效应,针刺时间可影响经穴效应,慢频率下应用较长针刺时间可相对获得更好效应,其内在作用机制可能是改善脑组织微循环状态、调节缺血后炎症反应,减轻神经细胞坏死。 |
| 关键词: 内关 针刺时间 效应 大脑中动脉缺血模型 |
| DOI：10.11656/j.issn.1672-1519.2013.09.13 |
| 分类号: |
| 基金项目:国家重点研究计划(973)资助项目(2010CB530506,2012CB518505);天津市应用基础与前沿技术研究计划资助项目(10JCBJC12700);天津市高等学校科技发展基金计划项目(20090206);长江学者和创新团队发展计划资助 |
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| Experimental study on influence at different time for intervention in MCAO rats by stimulating Neiguan acupoint with slow frequency |
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YANG Sha1,2,3, FAN Xiao-nong1,2,4, LUO Ding5, ZHANG Ya-nan5, WEI Yuan-yuan5, ZHANG Hai-tao5, CHEN Jian-fei5, HE Ni-na5, CHANG Xiao-bo5, WANG Shu1,2,3, MENG Zhi-hong1, SHI Xue-min1
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1.Acupuncture Institute, The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China;2.Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin 300193, China;3.Tianjin Acupuncture Key Laboratory, Tianjin 300193, China;4.Three-level Laboratory of dose-effect relationship of the State Administration of TCM, Tianjin 300193, China;5.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To explore the influence of intervention at different time in MCAO rats by stimulating Neiguan acupoint with slow frequency. [Methods] The rats subjected to MCAO with suture method by Zea-Longa were randomly divided into seven group: normal group, sham operation group, model group, the non-acupuncture group, and stimulating Neiguan acupoint for a short time (5 seconds) group, stimulating Neiguan acupoint for a moderate time (60 seconds) group, stimulating Neiguan acupoint for a long time (180 seconds) group. The parameters of the three groups with simulation on Neiguan acupoint were fixed at 1 time/s, and the frequency was 5, 60, 180 seconds of acupuncture time respectively, and the acupuncture was performed once every 12 hours for 6 times. The effect indicators, including the normal animal nerve function, microcirculatory blood flow in the local area with ischemia, infarction rate of the brain tissue and the mechanism indicators, including the diameter of microcirculatory vascular input branches, diameter of the output branches, as well as the morphological indicators of the cells of brain tissue with HE staining were observed. In the model group observation was done immediately after modeling successfully, while in the later 4 groups the observation was performed 72 h after modeling. [Results] The improvement of the effect indicators in the three groups with simulation on Neiguan acupoint was better than the model group, the non-acupuncture group (P<0.05). Among the mechanism indicators the number of micrangium in cortex, hippocampus, striatum, the number of normal nerve cells (P<0.05) increased. As the improvement of each indicators the stimulation with a long time group was better. [Conclusion] Acupuncture on the Neiguan acupoint can get better effects. The acupuncture time can affect the Meridian effect. Under slow frequency the stimulation with longer acupuncture time can relatively get better effect; its intrinsic mechanism may be improving brain tissue microcirculation, regulating inflammatory reaction after ischemia and relieving the nerve cell necrosis. |
| Key words: Neiguan acupuncture time effect MCAO |
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