生肌象皮膏对糖尿病大鼠溃疡愈合中VCAM-1与ICAM-1的影响

Effect of Shengji Xiangpi plaster on expression of VCAM-1 and ICAM-1 in rats with diabetic ulcer in wound healing

关键词:  生肌象皮膏  糖尿病溃疡  免疫-炎症反应  血管细胞黏附分子-1  细胞间黏附分子-1

Key words:  Shengji Xiangpi plaster  diabetic ulcer  immune-inflammatory response  VCAM-1  ICAM-1

摘要:

[目的] 探讨生肌象皮膏促进糖尿病大鼠溃疡创面愈合的免疫-炎症反应机制。[方法] 将Wistar大鼠随机分为4组，1组为正常对照组，其余3组采用STZ尾静脉注射制备糖尿病模型，4组动物均于背部打孔，造成溃疡模型。3组糖尿病溃疡大鼠按照血糖和体质量分层随机分为糖尿病对照组、凡士林组、生肌象皮膏组。生肌象皮膏组外用生肌象皮膏纱条外敷，凡士林组用凡士林纱条外敷，正常对照组、糖尿病对照组均以生理盐水纱条外敷，观察创面组织血管细胞黏附分子（VCAM-1）与细胞间黏附分子-1（ICAM-1）表达水平的变化。[结果] 生肌象皮膏组大鼠糖尿病溃疡创面的愈合速度加快，与糖尿病对照组比较于第7、14、21、30天有统计学差异；生肌象皮膏组大鼠溃疡创面中VCAM-1表达水平与糖尿病对照组比较于第14、21、30天降低，有统计学差异（P<0.05），与凡士林组比较第14、21、30天降低，有统计学差异（P<0.05）；糖尿病对照组大鼠溃疡创面中VCAM-1表达水平与正常对照组比较于第21、30天升高，有统计学差异（P<0.05）；生肌象皮膏组大鼠创面组织ICAM-1表达水平与糖尿病对照组比较于第14、21、30天降低，有统计学差异（P<0.01），与凡士林组比较第21、30天降低，有统计学差异（P<0.05）；与正常对照组比较，糖尿病对照组大鼠创面组织ICAM-1表达水平于第14、21、30天升高，有统计学差异（P<0.05）。[结论] 生肌象皮膏促进糖尿病大鼠溃疡创面的愈合与其调节免疫-炎症反应有密切联系。

Abstract:

[Objective] To investigate the mechanisms of the immune-inflammatory response promoted by Shengji Xiangpi plaster (SXP) in the process of ulcer healing in diabetic rats. [Methods] Wistar rats were randomly divided into four groups: one was served as normal control group and the remaining three groups rats were injected with STZ into tail vein for inducing diabetic. All the rats in this four groups were punched in the back for establishing the model of diabetic ulcer. The rats with diabetic ulcer in the three group were randomly stratified into diabetic control group, vaseline group and SXP group according to the blood glucose and body weight. The rats in SXP group were treated with SXP gauze compress externally, those in vaseline group treated with vaseline gauze compress and those in normal control group and diabetic control group were treated with physiological saline. The changes of VCAM-1 and ICAM-1 expression in the wound tissue were then observed. [Results] The diabetic ulcer wounds in the back of the rats in SXP group healed faster than those in the rats in diabetic control group and vaseline group. The difference was significant in 7th, 14th, 21th and 30th day compared with diabetic control group respectively. The expression of VCAM-1 in the rats in SXP group was lower than those in the rats in diabetic control group on the 14th, 21th and 30th day with significant difference. It was also lower than those in the rats in vaseline group on the 14th, 21th and 30th day with significant difference. The expression of VCAM-1 in rats of diabetic control group were higher than those in the rats of normal control group on the 21th and 30th day and the difference between them was significant; the expression of ICAM-1 in the rats in SXP group were lower than those in the rats of diabetic control group on the 14th, 21th and 30th day with significant difference; it were also significantly lower than those in the rats in vaseline group on the 21th and 30th day. The expression of ICAM-1 in the rats of diabetic control group were also significantly higher than those in the rats of normal control group on the 14th, 21th and 30th day. [Conclusion] Shengji Xiangpi plaster can promote the ulcer healing in diabetic rats, and it is closely related to regulating the immune-inflammatory response.