麻杏石甘汤对哮喘大鼠肺组织中Sec14l3表达的影响

Influence of Maxing Shigan decoction on expression of Sec14l3 in lung tissue of rats with asthma

关键词:  麻杏石甘汤  哮喘  Sec14l3  气道炎症  气道上皮细胞

Key words:  Maxing Shigan decoction  asthma  Sec14l3  airway inflammation  airway epithelial cell

摘要:

[目的] 研究麻杏石甘汤及其主要成分对哮喘模型大鼠肺组织中Sec14l3表达的影响。[方法] 70只大鼠随机分为空白组、模型组、地塞米松组、麻杏石甘汤组、麻黄组、杏仁组、甘草组，每组10只，第0、7天腹腔注射卵蛋白致敏，第14天开始雾化并给予药物，连续两周。最后一次雾化24 h后测肺泡灌洗液（BALF）中炎症细胞数、病理观察气道炎症变化、实时定量聚合酶链反应（PCR）检测大鼠肺组织中Sec14l3 mRNA的水平。[结果] 与正常组相比，模型组BALF中白细胞总数、嗜酸细胞、中性粒细胞数明显升高，肺组织中可见大量炎性细胞浸润及气道上皮细胞损伤，Sec14l3 mRNA表达量明显减少，有统计学意义差异；与模型组相比，麻杏石甘汤组、地塞米松组、甘草组BALF中白细胞总数、嗜酸细胞、中性粒细胞数目明显减少，肺组织中未见大量炎性细胞浸润及上皮细胞损伤，Sec14l3 mRNA量明显增加，差异有统计学意义，而麻黄组、杏仁组的上述各项指标与模型组相比无统计学意义差异。[结论] 麻杏石甘汤及甘草能够有效减轻哮喘大鼠气道炎症的程度，其机制可能与调控Sec14l3在肺组织的表达水平，增强气道保护作用有关。

Abstract:

[Objective] To study the influence of Maxing Shigan Gan decoction(MXSGD) and its main parts on the expression of Sec14l3 in the lung tissue of rat with asthma. [Methods] Seventy Wister rats were randomly divided into seven groups: normal group, model group, dexamethasone group, MXSGD group, Mahuang group, Xingren group, Gancao group, with 10 rats in each group. Rats were sensitized by intraperitoneal injection of ovalbumin on 0th and 7th day, followed by challenge with aerosolized ovalbumin on 14th day for two weeks and drugs administration. 24 h after allergen challenge, the inflammatory cells of BALF was tested. The inflammatory alteration of airway was observed through pathological slices and the expression quantity of the Sec14l3 mRNA in rat tissues was measured by RT-PCR. [Results] Compared with the normal group, the number of total cells, Eosinophils and Neutrophils in model group were increased obviously. The histopathological alteration of lung tissue exhibited serious inflammatory cell infiltration and airway epithelial injury. The Sec14l3 mRNA decreased substantially. Compared with model group, the number of total cells, Eosinophils and Neutrophils in MXSGD group, dexamethasone group, Gancao group decreased obviously. Seldom inflammatory cell infiltration and airway epithelial injury existed in lung tissue, and the Sec14l3 mRNA increased significantly, while Mahuang group and Xingren group had no difference with the model group. [Conclusion] MXSGD can effectively reduce the inflammatory cell infiltration and airway epithelial injury in the lung of rat with asthma, and the mechanism may be related to the regulation of Sec14l3 in the lung tissue and improvement of the protection against the airway epithelia.