芎蚓中风颗粒的质量标准研究

Study on quality standard for Xiongyin Zhongfeng granules

关键词:  芎蚓中风颗粒  薄层鉴别  阿魏酸  芍药苷  含量测定

Key words:  Xiongyin Zhongfeng granules  TLC  Ferulic acid  Paeoniflorin  content determination

摘要:

[目的] 建立芎蚓中风颗粒的质量标准。[方法] 采用薄层色谱法对其进行定性分析。采用高效液相色谱（HPLC）法测定其中阿魏酸和芍药苷的含量。阿魏酸：C18色谱柱（250 mm×4.6 mm，5 μm）；甲醇-1%醋酸溶液（35：65）为流动相；流速为1 mL/min；检测波长为321 nm.芍药苷：C18色谱柱（250 mm×4.6 mm，5 μm）；乙腈-0.01%磷酸溶液（15：85）为流动相；流速为1 mL/min；检测波长为230 nm.[结果] 建立了该药中川芎、当归、地龙、赤芍的鉴别方法。阿魏酸在4.286～42.86 μg/mL（r=1.000）线性关系良好，平均回收率为99.7%.RSD=2.8%（n=6）.芍药苷在0.058 72～0.587 2 mg/mL（r=0.999 99）线性关系良好，平均回收率为99.5%，RSD=0.67%（n=6）.[结论] 该方法简便、可靠、重复性好，可用作芎蚓中风颗粒的质量控制。

Abstract:

[Objective] To establish the quality standard for Xiongyin Zhongfeng granules. [Methods] A identification of Xiongyin Zhongfeng Granules was performed by TLC. The ferulic acid and paeoniflorin were determinated by HPLC. Ferulic acid: It was separated on C18(250 mm×4.6 mm,5μm)column. The mobile phase was methanol-1% acetic acid solution(35:65). The flow rate was 1mL/min. The detection wavelength was 321nm. Paeoniflorin: It was separated on C18(250 mm×4.6 mm,5 μm)column. The mobile phase was acetonitrile-0.01% phosphate solution(15:85). The flow rate was 1 mL/min. The detection wavelength was 230 nm. [Results] Identification of ligusticum chuanxiong hort, angelica, pheretima,paeonia of Xiongyin Zhongfeng granules was established by TLC. The linear range of ferulic acid was 4.286～42.86 μg/mL(r=1.000).The average recovery was 99.7% with RSD=2.8%(n=6). The linear range of paeoniflorin was 0.058 72～0.587 2 mg/mL(r=0.999 99).The average recovery was 99.5% with RSD=0.67%(n=6).[Conclusion] These methods are simple, accurate and repeatable. They can be used as quality standard for Xiongyin Zhongfeng granules.