五味子复方减轻阿霉素肾病小鼠蛋白尿的实验研究

Experiment research of Schisandra Chinensis Mixture decreases proteinuria of mice with adriamycin-induced nephropathy

关键词:  五味子科  阿霉素肾病  Nephrin  Podocin  WT1  足细胞  蛋白尿

Key words:  schisandraceae  adriamycin-induced nephropathy  Nephrin  Podocin  WT1  podocyte  proteinuria

摘要:

[目的] 观察五味子复方（SM）对阿霉素肾病（ADN）小鼠肾皮质WT1蛋白、Nephrin和Podocin mRNA的影响，探讨SM减轻蛋白尿的可能机制。[方法] 雄性BALB/c小鼠27只随机分为对照组、ADN模型组和SM治疗组，单次尾静脉注射阿霉素法建立ADN模型，收集尿及肾组织，检测体质量、右肾质量、24 h尿白蛋白排泌率（UAER）；光镜下观察肾组织形态改变；免疫荧光检测各组肾小球WT1；逆转录-聚合酶链反应（RT-PCR）检测各组肾皮质Nephrin和Podocin mRNA.[结果] 治疗组的右肾质量、UAER低于模型组（均P<0.05）；治疗组Nephrin mRNA和Podocin mRNA表达水平高于模型组（均P<0.01）；肾小球荧光染色示WT1在对照组正常，模型组呈节段性缺失，治疗组缺失较轻。[结论] SM可以通过上调WT1蛋白、Nephrin和podocin mRNA的表达，保护足细胞，减少ADN小鼠的蛋白尿。

Abstract:

[Objective] Observating the effect of Schisandra Chinensis Mixture(SM) on the Wilms' tumor 1(WT1) protein and nephrin mRNA and podocin mRNA in renal of mice with ADN,to explore the mechanisms of SM decreases proteinuria. [Methods] Twenty-seven male Balb/c mice were randomly divided into three groups:normal control group, ADN group and SM group.The ADN was induced by a single tail-vein injection of ADR.The body weight, right kidney weight and UAER were measured. Furthermore, The morphological changes of renal tissue were observed under microscope. WT1 protein was observed by immunofluorescene.The mRNA of nephrin and podocin was determined by RT-PCR. [Results] Compared with the ADN group, there were significantly lower right kidney weight and UAER(P<0.05). There were significantly higher mRNA expression of nephrin and podocin in SM group than ADN group(P<0.01). WT1 was normal in control group,while it was focal absence in ADN group, and slightly absence in SM group. [Conclusion] After treatment with SM, WT1 protein, nephrin mRNA and podocin mRNA were up-regulated, which may safeguard podocyte, reduce proteinuria of mice with ADN.