| 摘要: |
| [目的] 建立同时测定金黄膏中盐酸小檗碱、巴马丁含量的方法。[方法] 采用高效液相色谱法，色谱柱为Apollo C18（250 mm×4.6 mm，5 μm），流动相为乙腈-0.1%磷酸（二元梯度洗脱），检测波长为 346 nm，流速为1.0 mL/min，柱温为30 ℃。[结果] 盐酸小檗碱和巴马丁的进样量分别在0.007 25～0.145 μg，0.005 5～0.110 μg范围内与各自峰面积积分值呈良好的线性关系，平均加样回收率分别为98.8%和100.9%.[结论] 该方法操作简便、重现性好，适用于金黄膏中巴马丁和盐酸小檗碱的含量测定。 |
| 关键词: 金黄膏 盐酸小檗碱 巴马丁 高效液相色谱法 |
| DOI：10.11656/j.issn.1672-1519.2014.02.12 |
| 分类号: |
| 基金项目:国家自然科学基金面上项目（81072818）. |
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| Determination of berberine and palmatine in Jinhuang cream by HPLC |
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LIU Jun-hong1, WANG Hong2, LI Di-hua1, QU Peng-fei2, LV Yuan-shan1
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1.Tianjin Institute of Acute Abdomen by Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China;2.The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China
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| Abstract: |
| [Objective] To establish a method for simultaneous determination of berberine and palmatine in Jinhuang cream. [Methods] A HPLC method was performed on Apollo C18 (250 mm×4.6 mm, 5 μ) column. The mobile phase consisted of acetonitrile-0.1% phosphoric acid(gradient elution)with the detection wavelengh at 346 nm. The flow rate was 1.0 mL/min and the column temperature was at 30 ℃. [Results] The content of berberine hydrochloride in the range of 0.007 25~0.145 μg and the content of palmatine in the range of 0.005 5~0.110 μg had good linear relationships with the area of eluted peak,and the recovery rate(the average recoveries) was 98.8% and 100.9% respectively. [Conclusion] This method is simple and repeatable, and is suitable for the content determination of berberine and palmatine in Jinhuang cream. |
| Key words: Jinhuang cream berberine hydrochloride palmatine HPLC |
