| 摘要: |
| [目的] 探讨参附注射液对局灶性脑缺血大鼠Na-K-Cl协同转运蛋白1（NKCC1）的影响。[方法] 雄性SD大鼠随机分为假手术组（Sham），缺血模型组（Ischemia）和缺血后给予参附注射液治疗组（SFI）.利用线栓法制作脑缺血/再灌注模型，再灌注3 h及24 h后进行神经功能评分，再灌注24 h后2，3，5氯化三苯基四氮唑蓝（TTC）法比较脑梗死面积，免疫组织化学及蛋白印迹法检测NKCC1的表达。[结果] 参附注射液治疗组的脑梗死面积、神经功能缺损程度均比对照组明显降低（P<0.05）.和对照组相比，SFI组的NKCC1表达明显低于Ischemia组（P<0.05）.[结论] 参附注射液对大鼠脑缺血模型的神经保护作用可能与抑制NKCC1相关。 |
| 关键词: 参附注射液 缺血性脑卒中 Na-K-Cl协同转运蛋白1 |
| DOI：10.11656/j.issn.1672-1519.2014.07.15 |
| 分类号: |
| 基金项目:教育部中央高校基金项目(JYBZZ－JS005），教育部留学回国人员科研启动基金。 |
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| Neuroprotective role and its mechanism of Shenfu injection on focal ischemic stroke in rats |
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YANG Yong1, REN Chang-hong2, WU Xiao-dan1
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1.Prescription Department, Beijing University of Chinese Medicine, Beijing 100029, China;2.Hypoxia Medical Research Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100053, China
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| Abstract: |
| [Objective] To investigate the effect of Shenfu injection on the expression of Na-K-Cl-cotransporter 1 (NKCC1) in rats with focal cerebral ischemic stroke. [Methods] The ischemic stroke was generated by 90 min occlusion of right middle cerebral artery. After 24 h reperfusion, neurological score was evaluated. The cerebral infarct volumes were quantified by 2, 3, 4-triphenytetrazolium-chloride (TTC), and the NKCC1expression were evaluated by Western blot and immunofluorescence methods. [Results] Shenfu injection-treatment group (SFI) significantly reduced the infarct volume (P<0.05) and the degree of neurological deficits (P<0.05). Compared to the ischemia group, the protein expression of NKCC1 was significantly reduced in the SFI group. [Conclusion] Shenfu injection plays a neuroprotective role in rats with experimental cerebral ischemia, which was associated with the inhibition of NKCC1. |
| Key words: Shenfu injection ischemic stroke Na-K-Cl-cotransporter 1 |
