涤痰散结活血软坚中药对非酒精性脂肪肝大鼠血清白介素-6、超氧化物歧化酶表达的影响

Effects on the traditional Chinese medicine of Ditan Sanjie, Huoxue Ruanjian on the expression of IL-6, SOD in rats with nonalcoholic fatty liver disease

关键词:  非酒精性脂肪肝  涤痰散结活血软坚法  白介素-6  超氧化物歧化酶

Key words:  nonalcoholic fatty liver disease  Ditan Sanjie, Huoxue Ruanjian method  interleukin-6  superoxide dismutase

摘要:

[目的] 通过观察涤痰散结活血软坚中药对非酒精性脂肪肝大鼠血清白介素-6（IL-6）、超氧化物歧化酶（SOD）表达的影响，探讨复方中药的作用机制。[方法] 雄性SPF级Wistar大鼠60只，按体质量分层随机分为4组：正常对照组14只，自由饮水和普通饲料饮食。模型对照组18只，中药高剂量组14只，中药低剂量组14只，均予自由饮水和高脂饲料饮食，8周后随机处死模型对照组大鼠4只，确定脂肪肝模型成立。造模成功后，分别对中药低剂量组和高剂量组行中药灌胃，模型对照组、正常对照组以生理盐水灌胃，每周称大鼠体质量，治疗8周后处死，采用ELISA法检测血清IL-6、SOD.[结果] 大鼠血清IL-6比较：高剂量组、低剂量组与模型对照组比较差异有显着性（P<0.01），高剂量组与低剂量组比较，差异有显着性（P<0.05）.大鼠血清SOD比较：高剂量组与模型对照组比较，差异有显着性（P<0.01），低剂量组与模型对照组比较差异有显着性（P<0.05），高剂量组与低剂量组比较无统计学意义（P>0.05）.[结论] 涤痰散结活血软坚中药可以显着减低血清IL-6浓度，此作用表现出剂量相关性趋势，可以明显升高血清SOD浓度，此作用没有体现出剂量相关性趋势。

Abstract:

[Objective] To establish a model of fatty liver induced by fat-rich diet in rats, observe the effect of the traditional Chinese medicine of Ditan Sanjie(clearing away phlegm and dispersing the accumulation), Huoxue Ruanjian(activating blood circulation and softening the hard mass) on the expression of IL-6, SOD and discuss the mechanism of Ditan Sanjie, Huoxue Ruanjian method. [Methods] Sixty male Wistar rats (SPF level) were randomly divided into 4 groups according to the weight: control group(n=14), fed normal diet; model group(n=18), fed fat-rich diet; traditional Chinese medicine high-dose group(n=14), fed fat-rich diet; traditional Chinese medicine low-dose group(n=14), fed fat-rich diet. Four of model control group were randomly killed 8 weeks later. Low-dose group and high-dose group were fed with Chinese herbs for 8 weeks. Model group and control group were fed with isotonic Na chloride. All of the rats were weighted every week, 8 weeks after treatment and all of the rats were killed. The serum levels of IL-6 and SOD were detected by ELISA method. [Results] The serum levels of IL-6 in rat of high-dose group and low-dose group showed highly significant difference compared with model group (P<0.01). The comparison between high-dose group and low-dose group showed significant difference (P<0.05). The serum levels of SOD in rats showed highly significant difference between high-dose group and model group (P<0.01) and showed significant difference between low-dose group and model group(P<0.05). The comparison of serum SOD showed no statistically significance between high-dose group and low-dose group (P>0.05). [Conclusion] Tthe traditional Chinese medicine of Ditan Sanjie, Huoxue Ruanjian can significantly decrease the serum levels of IL-6, and significantly increase the serum concentrations of SOD. However, this effect does not show any dose-related trend.