| 摘要: |
| [目的] 研究中药复方妇科灌肠Ⅱ号冻干粉对子宫内膜异位细胞雌激素(E2)及前列腺素E2(PGE2)分泌影响.[方法] 以Caco-2细胞单层模型为工具,模拟人体内中药灌肠法,将间质细胞和Caco-2细胞共培养,将妇科灌肠Ⅱ号及其他干预因素通过Caco-2单层细胞模型后作用于间质细胞.24 h后检测间质细胞培养液中E2和PGE2的浓度.[结果] 妇科灌肠Ⅱ号能降低异位间质细胞分泌的E2和PGE2浓度.[结论] 妇科灌肠Ⅱ号能通过干预子宫内膜异位症(EMS)局部病灶E2分泌来抑制疾病进一步发生发展. |
| 关键词: 子宫内膜位症 雌激素 前列腺素E2 妇科灌肠 Caco-2细胞模型 |
| DOI：10.11656/j.issn.1672-1519.2015.01.10 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(81072837). |
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| Effect of Fuke Guanchang II on secretion of estrogen and prostaglandin E2 from isolated cultured ectopic stromal cells |
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DAI Zhi-rong1, LI Pei-lin2, WANG Pan-pan1, ZHAO Zhi-mei2, LI Jie2, WANG Jian-ling2, ZHANG Shu-ying1
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
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| Abstract: |
| [Objective] To investigate the effects of Fuke GuanchangⅡrecipe (enema II for patients in gynecotokology) on secretion of E2 and prostag landin E2(PGE2) from ectopic endometrial cells. [Methods] Using the mono-layer model of Caco-2 cells as a tool, miming the enema method used in traditional Chinese medicine in human body, the endometrial stromal cells and Caco-2 cells were co-cultured together. The Fuke Guanchang II and other subjects factors were stimulated on the endometriosis ectopic endometrial cells through Caco-2 cell monolayer model. After 24 hours, the level of PGE2 and E2 secreted from endometrial cells was measured. [Results] The Fuke Guanchang II could reduce the E2 and PGE2 content, secreted by the ectopic endometrial cells. [Conclusion] The Fuke Guanchang II can inhibit the development of EMS through reducing the autocrine of E2 in the endometrial stromal cells. |
| Key words: endometriosis estrogen prostaglandin E2 Fuke Guanchang Caco-2 cell model |
