补肾中药有效成分对SAMP8小鼠氧化应激水平的影响

Effect of effective components of Chinese medicine for tonifying kidney on the levels of oxidative stress in SAMP8 mice

关键词:  补肾中药  老年性痴呆  补骨脂素  齐墩果酸  淫羊藿苷  氧化应激  SAMP8 小鼠

Key words:  kidney tonifying herb  senile dementia  psoralen  oleanolic acid  icariin  oxidative stress  SAMP8 mice

摘要:

[目的] 观察补肾中药有效成分补骨脂素、齐墩果酸、淫羊藿苷对快速老化(SAMP8)小鼠氧化应激水平的影响。[方法] 选取9 月龄雄性SMAP8 小鼠,采用随机数字表法随机分为补骨脂素组、齐墩果酸组、淫羊藿苷组及模型组。同时另取同龄段的抗快速衰老亚系1(杂粤酝砸员)小鼠作为对照组。治疗组分别给予补骨脂素(50 mg/kg)、齐墩果酸(50 mg/kg)、淫羊藿苷(20 mg/kg)灌胃,对照组与模型组均给予等量的生理盐水灌胃,每次0.3 mL,每日1 次,连续灌胃4周后断头取脑。采用酶联免疫吸附实验(ELISA)法检测SMAP8 小鼠大脑皮质中谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的含量。[结果] SMAP8 小鼠大脑皮质中SOD、CAT 和GSH-Px 活性较同龄SAMR1 小鼠明显降低,模型组大脑皮质SOD、CAT和GSH-Px 活性均低于对照组,经4 周治疗后,治疗组大脑皮质中SOD、CAT和GSH-Px 活性均高于模型组,对于以上指标,补骨脂素、齐墩果酸、淫羊藿苷3 种组间比较差异无统计学意义(P>0.05)。[结论] 补肾中药对SAMP8 的氧化应激损伤具有一定保护作用,从而可以达到防治老年性痴呆的作用。

Abstract:

[Objective] To observe the effect of effective components in tonifying kidney herbs of psoralen, oleanolic acid, icariin on oxidative stress level with mice (SAMP8). [Methods] This experiment chose september-aged male SMAP8 mice, according to the random number table method were randomly divided into psoralen group, oleanolic acid group, icariin group and model group. At the same time another age segment SAMR1 mice were chosen as control group. The treatment group were given psoralen (50 mg/kg), oleanolic acid (50 mg/kg), icariin (20 mg/kg) by gavage, saline control group and model group were given the same amount of gastric perfusion, 0.3 mL each time, 1 time a day, continuous intragastric administration for 4 weeks, then the rats were killed by decapitation and getting the brain. Using enzyme-linked immunosorbent assay (ELISA) method for detecting the contents of glutathion peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD) in cerebral cortex of SMAP8. [Results] SOD, CAT and GSH-Px activity in SAMR1 mice were decreased compared with age-matched of SMAP8 in the cerebral cortex; cerebral cortex SOD, CAT and GSH-Px activity in the model group were lower than the control group, after 4 weeks of treatment, SOD, CAT and GSH-Px activity in the cerebral cortex of the treatment group were higher than those in the model group, and there was no significant difference between psoralen, oleanolic acid, icariin three groups of the above indicators (P>>0.05). [Conclusion] The kidney tonifying herbs has certain protective effect on oxidative stress injury in SAMP8, so as to achieve the effect of prevention and treatment of senile dementia.