四君子汤对肠梗阻解除后肠黏膜屏障功能影响的实验研究

Effects of early application of Sijunzi decoction on intestinal mucosal barrier after relieving obstruction

关键词:  四君子汤  肠黏膜屏障  肠功能障碍  内毒素  肠黏膜损伤评分

Key words:  Sijunzi decoction  intestinal mucosal barrier  intestinal dysfunction  endotoxin  Chiu intestinal mucosa injury score

摘要:

[目的] 利用可复性机械性完全性肠梗阻模型,观察肠梗阻解除后自然恢复及中药四君子汤对肠屏障功能的影响。[方法] 选用健康大耳白兔,随机分为正常对照组、梗阻2 d组、自然恢复2 d组、中药治疗2 d组、自然恢复4 d组、中药治疗4 d组,每组6只。对动物模型梗阻解除后2 d、4 d时内毒素、肠黏膜损伤评分、小肠上皮层黏膜完整性情况分别进行检测和观察。[结果] 结果表明在肠梗阻解除后,内毒素水平逐渐降低(P<0.05),且中药治疗组血浆内毒素水平以及Chiu 肠黏膜损伤评分明显低于自然恢复组(P<0.05),中药治疗组绒毛表面积明显高于自然恢复组(P<0.05)。[结论] 肠梗阻解除后,中药四君子汤可有效地修复损伤的肠黏膜上皮细胞,防止肠道菌群移位,降低血浆内毒素水平,具有保护肠黏膜屏障的功能。

Abstract:

[Objective] The changes of intestinal mucosal barrier after relieving obstruction was investigated. And the effects of early application of Sijunzi decoction on intestinal mucosal barrier after relieving obstruction was assessed. [Methods] The normal rabbits were randomly divided into 6 groups including control group, intestinal obstrution 2 d model group, natural recovery 2 d group, application of Sijunzi decoction 2 d group, natural recovery 2 d group, application of Sijunzi decoction 4 d group. Each group included 6 rabbits. After relieving obstruction the serum endotoxin, Chiu intestinal mucosa injury scores and the integrality of intestinal epithelium was observed by microscope in 2, 4 d. [Results] The level of endotoxin was reduced gradually after relieving obstruction. Moreover, the level of endotoxin and Chiu's histopathological evaluation score was obviously lower in the Sijunzi decoction groups than that in the natural recovery groups (P<0.05). The surface area of villus was obviously higher in the Sijunzi decoction groups than that in the natural recovery groups (P<0.05). [Conclusion] The Sijunzi decoction can efficiently restore the lesion of intestinal mucous epithelium to block bacterial translocation and to lower the level of endotoxin. Hence, it can obviously protect intestinal mucosal barrier function.