消岩汤剂拆方配伍对A549肺腺癌细胞体外生长抑制作用研究

Study of the inhibition effect and mechanism of Xiaoyan decoction and decomposed recipe on A549 cells in vitro

关键词:  消岩汤  肺腺癌A549细胞  体外抑制作用

Key words:  Xiaoyan decoction  A549 lung adenocarcinoma cancer cell  inhibition in vitro

摘要:

[目的] 探讨消岩汤及各拆方组对人肺腺癌 A549细胞的体外生长的作用机制,探究消岩汤剂中最有效的作用成分和浓度。[方法] 消岩汤全方和根据功效拆分的清热解毒组、活血化瘀组、益气扶正组,以CCK-8检测A549肺腺癌细胞的细胞增殖活性。[结果] 各组均有明显的抑制细胞活性作用,抑制强度依次为:全方组、清热解毒组、活血化瘀组、益气扶正组。[结论] 消岩汤及其拆方组在体外实验中均能抑制A549肺腺癌细胞细胞生长,消岩汤全方组当浓度达到50 g/L时达到最大抑制率为85.60%。清热解毒组药物在抗肿瘤作用方面,与全方接近,提示清热解毒组中药为体外实验中全方抗肿瘤作用最有效组分。

Abstract:

[Objective] To investigate the inhibition of Xiaoyan decoction and decomposed recipe on A549 in vitro and its possible mechanism. Explore the most effective composition and concentration of Xiaoyan decoction. [Methods] According to the effect, Xiaoyan decoction was divided into three groups: Qingre Jiedu group, Huoxue Huayu group and Yiqi Fuzheng group. Use CCK 8 method to detect proliferation activity of A549 lung adenocarcinoma cancer cells. [Results] Groups had obvious inhibitory effect of cancer cell activity. The inhibition of strength in the order was Xiaoyan decoction group, Qingre Jiedu group, Huoxue Huayu group and Yiqi Fuzheng group. [Conclusion] Xiaoyan decoction and decomposed recipe groups have obvious inhibitory effect on A549 cells activity. Xiaoyan decoction (50 g/L) shows more inhibition on A549 cells with the lowest cell viability and is time-dependent. Effect of Qingre Jiedu group is close to Xiaoyan decoction, and suggests that Qingre Jiedu group is the most effective components for anti-tumor effect of the experiment in vitro of Xiaoyan decoction.