| 摘要: |
| [目的] 采用响应面法(RSM)优化川党参中多糖的提取工艺。[方法] 在单因素实验基础上,以提取温度、提取次数、提取时间3个因素为自变量,多糖提取率为响应值,采用三因素三水平的响应面分析各因素对响应值的影响。[结果] 多糖的最佳工艺条件为提取温度80 ℃,提取次数5次,提取时间5 h,多糖实际提取率可达22.31%。[结论] 应用响应面法优选出的提取工艺稳定、合理、可行。 |
| 关键词: 川党参 多糖 响应面法 提取工艺 |
| DOI：10.11656/j.issn.1672-1519.2015.07.13 |
| 分类号: |
| 基金项目:国家自然科学基金项目(31201951);辽宁省优秀人才项目(LJQ2013087). |
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| Optimization of the extraction technique of Codonopsis tangshen Dliv. polysaccharide using response surface methodoloy |
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WU Xin-liang1, WANG Hong-jun2, BAI Zi-xia1, LONG Yuan-tao2
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1.Department of Pharmacy, Tianjin Baodi Hospital, Tianjin 301800, China;2.Liaoning Medical University, Jinzhou 121001, China
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| Abstract: |
| [Objective] To optimize the extraction conditions of polysaccharide in Codonopsis tangshen Oliv. using response surface methodology (RSM). [Methods] On the basis of single factor test, extracting temperature, extracting times and extracting time were selected as response factors; the yield of polysaccharide was used as response value. A three-factor-three-level experiment design was developed. RSM was employed to analyze the results of experiments. [Results] The optimum extraction conditions of polysaccharide were as follows: extracting temperature was 80 ℃, extracting times was 5 times, and extracting time was 5 h. The yield of polysaccharide was 22.31%. [Conclusion] RSM employed in optimization of the extraction conditions is feasible. |
| Key words: Codonopsis tangshen Oliv. polysaccharide RSM extraction technique |
