黄芪注射液对高糖诱导的肾小管上皮细胞凋亡的影响

Influence of Astragalus on cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells

关键词:  糖尿病肾病  肾小管上皮细胞  黄芪注射液  细胞凋亡

Key words:  diabetic nephropathy  renal tubular epithelial cell  Astragalus injection  cell apoptosis

摘要:

[目的] 研究黄芪注射液对高糖环境下肾小管上皮细胞(HK-2)凋亡的影响。[方法] 传代培养人近曲肾小管上皮细胞,细胞用无血清培养基同步化24 h后,将细胞分为5组:低糖对照组、高糖组、高糖+不同浓度黄芪注射液组(2、20、200 μg/mL),每组设3个复孔。将细胞置于37 ℃恒温培养箱中培养至24、48、72 h,收集细胞及细胞上清液。用流式细胞仪测定细胞凋亡率。[结果] 24、48、72 h,低糖对照组细胞凋亡率明显低于高糖组(P<0.05)。细胞培养24 h,黄芪干预组200和20 μg/mL细胞凋亡率明显低于高糖组(P<0.05)。细胞培养48和72 h,黄芪注射液干预组细胞凋亡率明显低于高糖组(P<0.05)。黄芪注射液干预组各组之间凋亡率比较差异也都有显著性(P<0.05),其干预作用呈剂量依赖性。[结论] 黄芪注射液能抑制高糖诱导的肾小管上皮细胞的凋亡,有助于糖尿病肾病的治疗。

Abstract:

[Objective] To investigate the influence of Astragalus on cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells (HK-2). [Methods] Human renal proximal tubular cells were cultured and divided into five groups: low glucose as control group, high glucose group, Astragalus pretreatment group with concentration of 2, 20 and 200 μg/mL. Each group has three samples, cultured in 37 ℃ incubator for 24, 48 and 72 hours, and collected cells and cell supernatant fluid. Apoptosis rate was measured by flow cytometry. [Results] When cells were cultured 24, 48 and 72 h, and the apoptosis rate of control group were significantly lower than high glucose group (P<0.05). When cells were cultured 24 h, the apoptosis rate of 200 and 20 μg/mL group significantly lower than high glucose group (P<0.05). When cells were cultured 48 and 72 h, the apoptosis rate of 200, 20 and 2 μg/mL group significantly lower than high glucose group (P<0.05). The apoptosis were significant different between Astragalus injection groups (P<0.05), and the effect of Astragalus were showed in dose-dependent manner. [Conclusion] Astragalus can suppress cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells, which could be a novel approach of treating diabetic nephropathy.