调肝导浊方对apoE-/-小鼠血脂及主动脉斑块面积的影响

Effects of Tiaogan Daozhuo prescription on blood lipid and aorta plaque area in apoE-/- mice

关键词:  动脉粥样硬化  apoE^-/-小鼠  调肝导浊方  血脂  斑块

Key words:  atherosis  apoE^-/- mice  Tiaogan Daozhuo prescription  blood fat  plaque

摘要:

[目的] 观察调肝导浊方对apoE^-/-小鼠血脂及主动脉斑块的影响。[方法] 将apoE^-/-小鼠随机分成模型组和调肝导浊组并予以高脂饮食12周,C57BL/6L小鼠作为对照组给予普通饮食,12周后,检测各组小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)的水平;油红O染色评估主动脉斑块的范围;小动物超声仪检测主动脉的斑块面积及狭窄程度。[结果] 模型组与对照组相比,TC、TG、HDL-C、LDL-C均升高(P<0.05或P<0.01);调肝导浊组与模型组相比,LDL-C水平降低(P<0.05),TC、TG、HDL-C无明显差异。油红O染色显示模型组的斑块范围最大,其次是调肝导浊组,最后是对照组。B超结果显示与对照组相比,模型组主动脉的斑块面积及狭窄程度明显增大(P<0.01);调肝导浊方干预后,斑块面积减小(P<0.05),狭窄程度明显减轻(P<0.01)。[结论] 调肝导浊方可以降低apoE^-/-小鼠的血清LDL-C水平,减少主动脉的斑块面积,并改善血管的狭窄程度。

Abstract:

[Objective] To observe the effect of Tiaogan Daozhuo prescription on the blood lipid and aortic plaque in apoE^-/- mice. [Methods] ApoE-/- mice were randomly divided into model group and Tiaogan Daozhuo group, high fat diet for 12 weeks, C57BL/6L mice served as the control group were fed with normal diet, after 12 weeks, detection of serum TC, TG, HDL-C and LDL-C level, oil red O staining assessment aorta plaque scope; small animal ultrasound instrument to detect the aortic plaque area and degree of stenosis. [Results] TC, TG, HDL-C, LDL-C in the model group were higher than that in control group(P<0.05 or P<0.01). Compared with model group LDL-C decreased in Tiaogan Daozhuo group(P<0.05), and there was no significant difference of TC, TG and HDL-C. Oil red O staining showed that the range of plaque in the model group was the first, followed by the Tiaogan Daozhuo group, and the last was the control group. Compared with control group, the plaque area and stenosis of aorta in model group were significantly increased (P<0.01), intervention by Tiaogan Daozhuo prescription. The plaque area was decreased (P<0.05), and the degree of stenosis was significantly reduced (P<0.01). [Conclusion] Tiaogan Daozhuo prescription can decrease the serum levels of LDL-C in apoE^-/- mice, and decrease the aortic plaque area and improve the degree of vascular stenosis.