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| UPLC-Q-TOF/MS法鉴定大鼠体内甘草素的代谢产物 |
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李元元1,2, 江振作1,2, 张蕾1,2, 柴欣1,2, 王跃飞1,2
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457
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| 摘要: |
| [目的] 研究甘草素在大鼠体内的代谢产物。[方法] 采用静脉注射(iv)、灌胃(ig)两种给药方式,剂量20 mg/kg,收集大鼠血浆、尿液、粪便、胆汁,应用UPLC-Q-TOF/MS法鉴定甘草素的代谢产物。[结果] 血浆、尿液、粪便、胆汁样品中共鉴定15种代谢产物,主要有异甘草素、(异)甘草素葡萄糖醛酸结合物和硫酸结合物、二氢异甘草素、二氢异甘草素硫酸结合物、异甘草素甲基化及甘草素脱氢的代谢产物;其主要代谢途径为异构化、葡萄糖醛酸化、硫酸化、甲基化、脱氢反应。[结论] 构建的UPLC-Q-TOF/MS法可用于大鼠体内甘草素代谢产物的研究。 |
| 关键词: 甘草素 代谢产物 UPLC-Q-TOF/MS 异构化 葡萄糖醛酸化 硫酸化 |
| DOI：10.11656/j.issn.1672-1519.2015.12.14 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(81202877)。 |
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| Characterization of metabolites of liquirtigenin in rats by UPLC-Q-TOF/MS |
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LI Yuan-yuan1,2, JIANG Zhen-zuo1,2, ZHANG Lei1,2, CHAI Xin1,2, WANG Yue-fei1,2
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China
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| Abstract: |
| [Objective] To systemically study metabolites of liquirtigenin in rats, an UPLC-Q-TOF/MS method was developed. [Methods] UPLC-Q-TOF/MS method was employed to identify metabolites of liquirtigenin in plasma, urine, feces and bile samples from rats after iv and ig administration of liquirtigenin (20 mg/kg). [Results] A total of 15 metabolites were characterized in those biological samples, including isoliquirtigenin, glucuronides and sulfates of (iso)liquirtigenin, davidigenin and its sulfates, methylated isoliquirtigenin, dehydrogenated liquirtigenin. The main metabolic pathways of liquirtigenin were isomerization, glucuronidation and sulfation, methylation, dehydrogenation. [Conclusion] The UPLC-Q-TOF/MS method established in this study is successfully applied to identify metabolites of liquirtigenin in rats. |
| Key words: liquirtigenin metabolite UPLC-Q-TOF/MS isomerization glucuronidation sulfation |
