| 摘要: |
| [目的] 研究灯盏乙素对脂多糖（LPS）诱导的RAW264.7细胞炎症的影响，初步探讨其抗炎作用机制。[方法] 用噻唑蓝（MTT）法检测灯盏乙素对RAW264.7细胞活力的影响；用Griess法检测灯盏乙素对LPS诱导的RAW264.7细胞中一氧化氮（NO）生成的影响；用荧光素酶报告质粒法检测灯盏乙素对LPS诱导的核转录因子（NF-κB）转录活性的影响；用实时定量聚合酶链反应（PCR）法检测灯盏乙素对LPS诱导的RAW264.7细胞中NF-κB靶基因肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）、白介素-6（IL-6）mRNA表达的影响。[结果] 0.01～10 μmol/L的灯盏乙素处理24 h后对RAW264.7活力无显著影响；1、10 μmol/L的灯盏乙素可以显著抑制LPS诱导的NO的生成及NF-κB转录活性；灯盏乙素可以不同程度的抑制LPS诱导的RAW264.7细胞NF-κB靶基因TNF-α、IL-1β、IL-6 mRNA的上调作用。[结论] 灯盏乙素可能通过抑制NF-κB的活化及炎症因子的表达发挥一定的抗炎作用。 |
| 关键词: 灯盏乙素 炎症 RAW264.7细胞 作用机制 |
| DOI：10.11656/j.issn.1672-1519.2016.08.11 |
| 分类号: |
| 基金项目:国家科技支撑计划课题（2014BAI05B01）。 |
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| Effect of scutellarin on the secretion of inflammatory mediators of RAW264.7 cell induced by LPS |
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WEI Jing, CHEN Jing-rui, MIAO Lin, WANG Ya-dong, FAN Guan-wei
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Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the effect of scutellarin on RAW264.7 cell inflammation induced by LPS, and to explore the mechanism of its anti-inflammatory effect. [Methods] To detect effect of scutellarin on RAW264.7 cell viability by MTT, detect effect of scutellarin on NF-κB transcription activity induced by LPS, detect scutellarin on TNF-α, IL-1β, IL-6 mRNA expression of NF-κB target gene in RAW264.7 cell induced by LPS. [Results] It had no obvious significance on RAW264.7 by 0.01～10 μmol/L scutellarin treatment for 24 h; 1, 10 μmol/L scutellarin could significantly inhibit NO generation and NF-κB transcription activity induced by LPS; scutellarin could inhibit up-regulation of TNF-α, IL-1β, IL-6 mRNA of NF-κB target gene in RAW264.7 cell induced by LPS. [Conclusion] Scutellarin has certain anti-inflammatory effect by inhibition NF-κB activation and inflammatory factor expression. |
| Key words: scutellarin inflammation RAW264.7 cell mechanism |
