| 摘要: |
| [目的] 观察多重复合应激致慢性疲劳模型大鼠认知行为的变化，验证理虚解郁综合方对其认知的改善作用。[方法] 清洁级SD雄性大鼠80只，随机分组为对照组、模型组以及中药低、中、高剂量组，每组16只，除对照组外，均以复合因素造模（慢性束缚、冷水游泳、随机昼夜颠倒），同时中药低、中、高剂量组分以2 mL浓度为0.5、1、2 g/mL中药灌胃30 d，对照组和模型组则给予等体积的生理盐水。造模结束后应用行为学方法（旷场实验、悬尾实验及Morris水迷宫实验）检测不同组大鼠之间出现的差异表现。[结果] 与正常组比较，模型组大鼠在水迷宫实验中目标象限停留时间减少，穿越原平台的次数明显减少，旷场中移动距离和移动时间明显减少、而在活动箱边缘区域活动时间百分比增加，悬尾实验中静止时间延长、挣扎次数减少；中药三组与模型组比较，在水迷宫实验中，高剂量组在目标象限内停留的时间有显著增加，各剂量组穿越原平台的次数均明显增加；旷场实验中，中、大剂量组移动时间增加，大剂量组移动距离增加，中剂量组边缘区域活动时间百分比减少；悬尾实验中，中、大剂量组挣扎次数均增加，中剂量组静止时间缩短。[结论] 理虚解郁综合方能够明显改善慢性疲劳模型大鼠的认知行为学表现。 |
| 关键词: 中药 动物实验 行为学 |
| DOI：10.11656/j.issn.1672-1519.2016.09.10 |
| 分类号: |
| 基金项目:国家自然科学基金青年项目（81303099）；上海市杏林新星计划（ZY3-RCPY-2-2015）。 |
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| Intervention effects of Chinese herbal compounds on the behavior of chronic fatigue rats induced by combined stress |
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ZHANG Ye1,2, ZHANG Zhen-xian2, HUANG Yao2
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1.Scientific Research office, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;2.Medical Department, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medcine, Shanghai 200437, China
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| Abstract: |
| [Objective] To observe the changes of behavior of chronic fatigue rats induced by combined stress, and to verify the effect of Lixu Jiyu recipe on the improvement of behavior. [Methods] The 80 SD male rats were randomly divided into 5 groups:control group, model group, low dose treated group, middle dose treated group, and high dose treated group, 16 rats in each group. The CFS rats models were made with multiple stimuli including chronic restraint, Swimming in cold water and day and night reversed by randomly except control group. The rats in the control group and model group were given normal saline of 2 mL, and the treated groups were given concentration of 0.5, 1, 2 g/mL of Lixu Jieyu recipe for 30 days. To observe the rats'behavioristic changes in open-field test, tail hanging test, exhausting swimming test and morris water maze test among different groups. [Results] Compared with the control group, the model group manifested a series of abnormal variations in open-field test, tail hanging test. Compared with the model group, Lixu Jieyu recipe could improve moving time and distance in open-field test, reducing the percentage of active time of the marginal zone; increasing the times of struggle, shortening the time of rest in tail hanging test; prolonging the time of the rats in the target quadrant in Morris water maze test.[Conclusion] Lixu Jieyu recipe can significantly improve the behavior of rats with chronic fatigue. |
| Key words: traditional Chinese Medicine animal experiment behavioristics |
