豨红通络口服液改善小鼠炎性和神经病理性疼痛的作用研究

Inflammatory and neuropathic pain in mice reversed by Xihong Tongluo oral liquid

关键词:  豨红通络口服液  炎性肿胀  炎性疼痛  脑卒中  神经病理性疼痛

Key words:  Xihong Tongluo oral liquid  inflammatory swelling  inflammatory pain  stroke  neuropathic pain

摘要:

[目的] 探究豨红通络口服液对多种因素导致小鼠炎性、神经病理性疼痛的改善作用。[方法] 建立炎性肿胀模型、炎症性疼痛模型、扭体疼痛模型及脑卒中模型。进行豨红通络口服液灌胃（1次/日），然后在相应的时间点（1/48、1、3、5 d）进行炎性肿胀程度、疼痛行为变化以及受损脑组织病理损伤等计分评价。[结果] 给予豨红通络口服液低剂量（2 μL/g）、高剂量（10 μL/g）灌胃处理，随着给药时间的增加，耳廓肿胀、足趾肿胀程度与模型组相比得到了明显缓解，并且高剂量组治疗效果较好；同时苏木精-伊红（HE）染色显示豨红通络口服液灌胃处理能明显改善完全弗氏佐剂所诱导的炎性浸润；随着豨红通络口服液灌胃处理的天数增加，醋酸诱导的小鼠扭体次数显著减少，在给药第5天时高剂量治疗组治疗效果最为明显；脑组织Nissl染色发现，胶原酶导致脑卒中脑组织神经细胞着色变浅以及胶质细胞异常肥大，而给予豨红通络口服液灌胃处理的，神经细胞损伤以及胶质细胞异常肥大得到了较好缓解。[结论] 豨红通络口服液既能快速减轻起炎物质诱发的炎症肿胀和疼痛反应，也能改善脑卒中引起脑神经细胞受损及胶质细胞的异常肥大，有效治疗中枢性神经病理性疼痛，达到良好的镇痛效果。

Abstract:

[Objective] To investigate the effect of anti-inflammatory and neurophatic pain of Xihong Tongluo oral liquid.[Methods] A dimethyl benzene-induced mouse auris swelling model was established to observe anti-inflammatory effects. The inflammatory pain was induced by a subcutaneous injection of 50 μL CFA into the bottom of left hind paws of mice. The response to writhing and stretching reaction induced by acetic acid (HAc, 0.6%, 50 μL) injection into a peritoneal cavity of mice was evaluated. A central poststroke pain (CPSP) model was used with a single collagenase (type IV; 0.25 μL, 0.025 U) injection into the left ventral posterolateral nucleus of the thalamus. Xihong Tongluo oral liquid was administrated to mice via intragastric administration and saline was administrated as a control. Then at corresponding time points (1/48 d, 1 d, 3 d, 5 d), inflammatory swelling, pain related-behaviors and histopathology were conducted and evaluated blindly.[Results] The experimental results of ear swelling showed that Xihong Tongluo oral liquid (low-dosage:20 L/g; high-dosage:100 L/g) exerted positive regulation in a time-dependent manner, in a comparison with model group. On the fifth day, ear edema of mice in the high-dose group was significantly alleviated compared with other groups. The edema of hind paw in CFA-inflamed mice was obvious on the first day. While significant reductions were seen in the group intragastric administrate with Xihong Tongluo oral liquid, while the high-dosage group showed the best anti-inflammatory effect; HE staining revealed that gavage treatment of Xihong Tongluo oral liquid can significantly alleviated the CFA-induced inflammatory infiltration. With gavage treatment, the number of writhing in HAc groups was significantly reduced, and high-dosage group showed significant analgesic effect on the 5th day. Nissl staining showed that CPSP weakened Nissl body staining and increased the amount of hypertrophic glial cells.[Conclusion] Those symptoms were improved after gavage treatment of Xihong Tongluo oral liquid. Xihong Tongluo oral liquid can relieve inflammatory swelling and pain response. While the injured neuron and hypertrophic glial cells induced by CPSP were improved, Xihong Tongluo oral liquid exerted greater anti-inflammatory and analgesic effects on central neuropathic pain.