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甘露醇引发处理对丹参种子萌发及幼苗生长的影响
张玉, 石晓晨, 马云, 席晓志, 戚莹雪, 蒲高斌, 李佳, 刘谦, 张永清
山东中医药大学, 济南 250355
摘要:
为考察干旱胁迫处理对丹参种子萌发及幼苗生产的影响,文章以甘露醇作为引发剂,采用5个引发浓度(1%、3%、5%、7%、9%)和5个引发时间(0、4、8、12、16 h)对丹参种子进行了发芽试验,测定了种子发芽率、发芽势、简化活力指数等指标。结果显示,甘露醇不同引发浓度与引发时间对丹参种子的发芽势、发芽率、苗高、苗根长及简化活力指数均有一定影响,总体趋势是引发浓度越高、引发时间越长,对丹参种子萌发及幼苗生长的抑制作用越强,部分处理有显著提升简化活力指数的作用,以5%甘露醇引发4 h处理可明显提高丹参种苗质量,为丹参药材产量与质量的提高奠定了基础。
关键词:  丹参  种子  甘露醇  发芽势  发芽率  简化活力指数
DOI:10.11656/j.issn.1672-1519.2018.08.20
分类号:R932
基金项目:国家中医药管理局科研基金项目(ZYBZH-C-JX-38)。
Effect of mannitol initiation treatment on seed germination and seedling growth of Salvia miltiorrhiza
ZHANG Yu, SHI Xiaochen, MA Yun, XI Xiaozhi, QI Yingxue, PU Gaobin, LI Jia, LIU Qian, ZHANG Yongqing
Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Abstract:
In order to investigate the effect of drought stress treatment on the germination and seedling production of Salvia miltiorrhiza, mannitol was used as initiator in this study. Five induced concentrations (1%, 3%, 5%, 7%, 9%) and five time points (0 h, 4 h, 8 h, 12 h, 16h) germination tests on Salvia miltiorrhiza seeds were carried out. The seed germination rate, germination potential and simplified vitality index were measured. The results showed that different concentrations and time of mannitol had some effects on the germination potential, germination rate, seedling height, seedling root length and simplified vitality index of Salvia miltiorrhiza seeds. The general trend was that the higher the priming concentration and the longer priming time, Salvia miltiorrhiza seed germination and seedling growth inhibition, the stronger part of the treatment significantly improved the role of simplified vitality index to 5% mannitol 4h treatment can significantly improve the quality of Salvia seedlings, Salvia the yield and quality of the foundation.
Key words:  Salvia miltiorrhiza  seeds  mannitol  germination potential  germination rate  simplified vitality index
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