交泰丸含药血清对豚鼠心室肌细胞ICa-L的影响

Effect of Jiaotai Pills and its disassembled prescription on ICa-L in single guinea-pig ventricular myocytes

关键词:  交泰丸  黄连  肉桂  L-型钙离子电流  血清药理学  动力学参数

Key words:  Jiaotai Pill  Rhizoma Coptidis  Cortex Cinnamomi Cassiae  ICa-L  serum pharmacology  kinetic parameter

摘要:

[目的]观察交泰丸及其拆方含药血清对豚鼠心室肌细胞L-型钙离子通道（ICa-L）的影响。[方法]急性分离心室肌细胞，实验分为空白组、黄连组、肉桂组、黄连加肉桂组和交泰丸组，各组加入含药血清浓度为10%和15%，后放于37℃、5% CO₂的孵育箱中孵育24 h，运用膜片钳技术观察药物对ICa-L电流密度和动力学参数的影响。[结果]各组均不同程度抑制钙离子电流（P<0.05），使ICa-L激活和失活时间常数及失活后恢复时间延长（P<0.05）。其中交泰丸对ICa-L平均电流密度抑制率最高达57.5%，肉桂显著延长ICa-L激活时间常数和失活时间常数，黄连使ICa-L失活后恢复时间常数减小最显著。[结论]交泰丸及各拆方含药血清对钙离子通道有不同程度的抑制作用，交泰丸抑制率最大，抑制钙电流影响心肌细胞有效不应期，这可能是交泰丸在临床及实验中具有抗心律失常作用的机制。

Abstract:

[Objective] To explore the effect of Jiaotai Pills and its disassembled prescription in drug serum on ICa-L in guinea-pig ventricular myocytes.[Methods] Acute isolated guinea pig ventricular muscle cells and explore the effect of drug on ICa-L current and and channel dynamics parameters. The myocytes were randomly divided into five groups:the control group, the Rhizoma Coptidis group (decoction of 100 g goldthread), the Cortex Cinnamomi Cassiae group (decoction of 10 g cinnamon), the Rhizoma Coptidis and Cortex Cinnamomi Cassiae group(single decoction of 100 g goldthread + equal volume decoction of 10 g cinnamon), the Jiaotai Pills group (co-decoction of 100 g goldthread and 10 g cinnamon). Cells were divided into 10% and 15% drug concentration groups and incubated at 37℃ in ahumidified, 5% CO₂ incubator after 24 h, patch clamp test was performed.[Results] Compared with the control group, the drug groups (10%, 15%) inhibited ICa-L density to different degrees (P<0.05), and the highest inhibition rate of 10% concentration Jiaotai Pills was 57.5%. All groups made the activation, inactivation and recovery time contant prolonged (P<0.05). The rate of activation and inactivation time constant of Cortex Cinnamomi Cassiae group was the largest, and the rate of recovery time constant of Rhizoma Coptidis group was the largest.[Conclusion] Drug containing serum of Jiaotai Pills and disassembled prescription have different degrees of inhibition on calcium ion channels, and the mechanism of action are same with calcium blockers, it could be the mechanism of Jiaotai Pills antiarrhythmic.