基于HPLC-LTQ/Orbitrap MS法的四味脾胃舒颗粒化学成分研究

Analysis of chemical constituents by HPLC-LTQ/Orbitrap MS of Siwei Piweishu Granule

关键词:  HPLC-LTQ/Orbitrap MS  四味脾胃舒颗粒  化学成分

Key words:  HPLC-LTQ/Orbitrap MS  Siwei Piweishu Granule  chemical composition

摘要:

[目的]应用高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱（HPLC-LTQ/Orbitrap MS）技术鉴定四味脾胃舒颗粒中的化学成分，为四味脾胃舒颗粒的深入研究开发提供更多科学依据。[方法]通过查阅文献，收集整理四味脾胃舒颗粒处方中各味药材（茯苓、鸡屎藤、山楂、陈皮）及其同科属植物各类化学成分数据。采用HPLC-LTQ/Orbitrap MS法采集数据后，对各色谱峰的质谱图高分辨一级和二级质谱数据进行解析，与文献数据进行对比，对各色谱峰进行结构推测和确认。[结果]从四味脾胃舒颗粒样品中共分析出30个成分，鉴定了26个化合物，分别为黄酮类成分（橙皮素、柚皮素、刺芒柄花素）、苷类成分（Cyanidin 3-galactoside、野樱苷、芹菜苷）、酸类成分（去氢土莫酸、没食子酸）、生物碱类成分（刺桐碱、3-formylindole）。[结论]实验所建立的HPLC-LTQ/Orbitrap MS法适合于鉴定中药复方制剂四味脾胃舒颗粒主要化学成分，在一定程度上揭示了四味脾胃舒颗粒的药效物质物质基础。

Abstract:

[Objective] Analysis and identification chemical constituents in Siwei Piweishu Granule by HPLC-LTQ/Orbitrap MS. To provides scientific basis for further research and development of Siwei Piweishu Granule.[Methods] Through literature review,to collect the data of all kinds of chemical constituents in the prescription of Siwei Piweishu Granule. Data is collected by HPLC-LTQ/Orbitrap MS,High resolution MS and MS2 spectra of mass spectrogram of chromatographic peaks are analysised. Compare with the literature database. The structure of each chromatographic peak was calculated and confirmed.[Results] Twenty-six were identified from Siwei Piweishu Granule. The main constituents are Flavonoids (hesperetin,naringenin,formononetin);glycosides (cyanidin 3-galactoside,prunasin,apiin);acids(dehydrotumulosic acid,gallic acid);Alkaloids (hypaphorine,3-formylindole).[Conclusion] In this paper,HPLC-LTQ/Orbitrap MS was suitable to determine and analyze the mainly chemical composition of Siwei Piweishu Granule. To a certain extent,the pharmacodynamic material basis of Siwei Piweishu Granule was revealed by this research.