青蒿琥酯通过诱导抗氧化酶活性抑制PC12细胞氧化损伤

Protective effects of artesunate against PC12 cells with oxidative injury through inducing the activities of antioxidase

关键词:  青蒿琥酯  PC12细胞  活性氧  抗氧化酶

Key words:  artesunate  PC12 cells  reactive oxygen species  antioxidant enzyme

摘要:

[目的]研究青蒿琥酯对过氧化氢（H₂O₂）诱导PC12细胞氧化损伤的保护作用，并对其作用机制进行初探。[方法]CCK8法检测青蒿琥酯对PC12细胞相对存活率的影响，利用H₂O₂诱导PC12细胞建立氧化损伤模型，并通过CCK8法及乳酸脱氢酶（LDH）比色法分析青蒿琥酯保护PC12细胞免受H₂O₂损伤的效果，对比分析不同处理组间PC12细胞中活性氧（ROS）、丙二醛（MDA）含量及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性。[结果]检测剂量范围内青蒿琥酯对PC12细胞相对存活率无明显影响（P>0.05）；与H₂O₂诱导组相比，青蒿琥酯干预组细胞相对存活率明显升高（P<0.05），LDH、SOD及MDA水平明显降低（P<0.05），PC12细胞中SOD、CAT及GSH-PX活性显著升高（P<0.05），并均表现出浓度依赖性。[结论]0.1~1.6 mmol/L浓度范围内的青蒿琥酯对PC12细胞相对存活率没有影响，可以通过减少H₂O₂产生的活性氧保护PC12细胞。

Abstract:

[Objective] To investigate the protective effects of artesunate on PC12 cells with H₂O₂-induced oxidative damage and study its possible mechanisms.[Methods] CCK8 assay was used to assess the cell relative survival rates of PC12 cells induced by H₂O₂. Lactate dehydrogenase (LDH) assay was used to measure the protective effect of artesunate on PC12 cells injury induced by H₂O₂ as well. Malondialde (MDA),reactive oxygen (ROS),superoxide dismutase (SOD),CAT,GSH-PX in cells were detected by spectroscopy.[Results] Artesunate had no effect on relative survival rates of cultured PC12 cells under the tested concentrations. Compared with H₂O₂-induced group,the cell relative survival rates,the activities of SOD,CAT and GSH-PX were significantly increased in artesunate groups (P<0.05);while the levels of LDH,SOD and MDA were greatly decreased in artesunate groups compared with H₂O₂-induced group (P<0.05) with a dose-dependent manner.[Conclusion] Artesunate in a concentration range of 0.1~1.6 mmol/L can act as an antioxidant to prevent the cellular injury induced by H₂O₂ in cultured PC12 cells without affecting the cell survival rates.