不同绝经时期妇女雌激素受体亚型在阴道组织中的表达

Expression of estrogen receptor subtypes in vagina in different menopausal women

关键词:  绝经  雌激素受体  外阴阴道萎缩

Key words:  menopause  estrogen receptor  vulvovaginal atrophy

摘要:

[目的]评估雌激素受体（ER）在育龄期、围绝经期、绝经后期妇女阴道组织中的表达，为绝经泌尿生殖道疾病的治疗提供帮助。[方法]收集2016年11月-2017年11月因妇科良性疾病行阴式或腹式全子宫切除术的61例（育龄期24例，围绝经期18例，绝经后期19例）患者的阴道前壁组织，采用实时荧光定量聚合酶链式反应（qPCR）及免疫组化（IHC）方法检测3组患者阴道前壁组织中ERα、ERβ mRNA及蛋白的表达。[结果]3组阴道壁组织中均有ERα和ERβ表达，而且在3组中ERα和ERβ的表达存在显著差异。围绝经期及绝经后期妇女ERα的表达水平显著低于育龄期妇女（P<0.05），而且绝经后妇女ERα的表达水平显著低于围绝经期妇女（P<0.05）；而围绝经期及绝经后期妇女ERβ的表达水平显著高于育龄期妇女（P<0.05），同时绝经后妇女ERβ的表达水平显著高于围绝经期妇女（P<0.05）。[结论]绝经影响阴道组织中ER的表达，育龄期正常的阴道组织到围绝经期及绝经后期萎缩的阴道组织中ER的表达差异可能与绝经雌激素水平下降有关。

Abstract:

[Objective] Menopause due to decreased levels of estrogen,causing various physiological and pathological changes in the human body,including vaginal tissue. To evaluate the expression of estrogen receptor (ER) in the vaginal tissues of women of childbearing age,perimenopausal and postmenopausal women,and to provide assistance for the treatment of menopausal urogenital diseases.[Methods] The 61 cases of vaginal or abdominal hysterectomy for benign gynecological diseases between November 2016 and November 2017 were collected (24 in childbearing age,18 in postmenopausal women and 19 in postmenopausal women). Anterior wall tissue was used to detect the expression of ERα and ERβ mRNA and protein in the anterior vaginal tissue of the three groups using real. time quantitative polymERαse chain reaction (QPCR) and immunohistochemistry (IHC).[Results] The expressions of ERα and ERβ were found in the three groups of vaginal wall tissues,and there were significant differences in the expression of ERα and ERβ in the three groups. The expression level of ERα in perimenopausal and postmenopausal women was significantly lower than that in women of childbearing age (P<0.05),and the expression level of ERα in postmenopausal women was significantly lower than that in perimenopausal women (P<0.05);perimenopause and menopause. The expression level of ERβ in women was significantly higher than that in women of childbearing age (P<0.05),and the expression level of ERβ in postmenopausal women was significantly higher than that in perimenopausal women (P<0.05).[Conclusion] Menopause affects the expression of ER in vaginal tissue,and the difference in the expression of ER in vaginal tissues from normal vaginal period of childbearing age to atrophic vaginal tissue during postmenopausal and late menopause may be related to the decrease of menopausal estrogen levels.