参麦注射液抗氧化活性体外实验研究

In vitro study on the antioxidant activities of Shenmai Injection

关键词:  抗氧化  参麦注射液  DPPH自由基  一氧化氮自由基  过氧化氢  亚铁离子

Key words:  antioxidant  Shenmai Injection  DPPH free radical  Nitric oxide free radical  hydrogen peroxide  ferrous ion

摘要:

[目的] 系统研究参麦注射液体外抗氧化性能。[方法] 采用铁还原抗氧化能力 （FRAP）法、1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除法、一氧化氮（NO）自由基清除法、过氧化氢（H₂O₂）清除法、亚铁（Fe²⁺）离子螯合法分别测试不同稀释倍率参麦注射液的总抗氧化能力、DPPH·清除活性、NO·清除活性、H₂O₂清除活性和Fe²⁺离子螯合活性，并将结果与0.1mg/mL 2，6-二叔丁基对甲酚（BHT）、10 mmol/L水溶性维生素E（Trolox）和10%柠檬酸的结果进行比较。[结果] 参麦注射液具有一定的体外抗氧化能力，总抗氧化能力和对自由基清除活性均呈浓度正依赖性；10倍稀释参麦注射液总抗氧化能力显著低于BHT和Trolox （P<0.01），但高于柠檬酸（P<0.01）；参麦注射液对不同自由基清除能力存在差异，其强弱顺序为：NO·[（54.4±6.6）%] > Fe²⁺离子螯[（42.6±3.2）%] > DPPH·[（36.2±2.8）%] > H₂O₂[（23.5±1.2）%]。[结论] 参麦注射液体外抗氧化活性研究将为其抗氧化药理学机制提供了实验依据。

Abstract:

[Objective] To systematic explore the antioxidant activities of Shenmai Injection. [Methods] The total antioxidant capability,DPPH· scavenging activity,NO· scavenging activity,H₂O₂ scavenging activity and Fe²⁺ chelating activity of the Shenmai Injection,which was diluted by different rates,were determined by Ferrric reducing antioxidant power assay (FRAR),DPPH· scavenging assay,NO· scavenging assay,H₂O₂ scavenging assay and Fe²⁺ chelating assay respectively. The results were compared with those of 0.1 mg/mL BHT,10 mmol/L Trolox and 10% citric acid. [Results] Shenmai Injection has certain antioxidant capacity in vitro. The total antioxidant capacity of Shenmai Injection was lower than that of BHT and Trolox (P<0.01),but higher than that of citric acid. The free radical scavenging activities of Shenmai Injection were positive concentration-dependence and were different for different free radical types. The order is NO·[(54.4±6.6)%] > Fe²⁺ ion[(42.6±3.2)%] > DPPH·[(36.2±2.8)%] > H₂O₂[(23.5±1.2)%]. [Conclusion] The in vitro experimental data of the antioxidant activities of Shenmai Injection will provide data support for its pharmacological mechanisms.