伸筋易骨法调节兔膝关节周围软组织张力影响软骨形态的研究

Study on the effect of Shenjin Yigu method on cartilage morphology by adjusting the tension of soft tissue around knee joint in rabbit

关键词:  伸筋易骨法  膝关节骨性关节炎  生物力学  软骨组织

Key words:  Shenjin Yigu method  knee osteoarthritis  biomechanics  cartilage tissue

摘要:

[目的] 评价伸筋易骨法对膝关节周围软组织张力及软骨形态的影响，以探讨其对膝关节骨性关节炎（KOA）的治疗作用。[方法] 选择6~8月龄雌性健康新西兰大耳白兔50只，随机分为5组。其中模型组、推拿组、玻璃酸钠组采用Hulth造模法模拟KOA，正常组、假手术组不做干预。造模成功后，推拿组予伸筋易骨法治疗3周，玻璃酸钠组予局部关节内注射每周1次，共3周，模型组不予任何治疗。治疗结束后，测试股直肌、股二头肌张力，并取软骨组织进行HE染色后光镜下进行形态学观察，并进行Markin评分。分析推拿组与其余4组的异同。[结果] 模型组较正常组，股直肌FDD值和S值显著降低（P<0.05），股二头肌FDD值和S值显著升高（P<0.05）。推拿组和玻璃酸钠组相较于模型组的股直肌FDD值和S值均显著升高（P<0.05））；推拿组和玻璃酸钠组相较于模型组股二头肌的FDD值和S值均显著降低（P<0.05）。推拿组和玻璃酸钠组相较于模型组，Mankin’s评分比较有统计学意义（P<0.05）。[结论] 伸筋易骨法可以显著增加关节周围肌肉的肌力、调节组织张力、改善关节活动功能，对保护膝关节周围软组织功能具有重要意义。

Abstract:

[Objective] To evaluate the effect of Shenjin Yigu method on soft tissue tension and cartilage morphology around knee joint,and to explore its therapeutic effect on knee osteoarthritis (KOA).[Methods] Fifty healthy female New Zealand big-ear white rabbits aged 6~8 months were selected and randomly divided into 5 groups. The model group,massage group and sodium hyaluronate group used Hulth modeling method to simulate KOA,while the normal group and sham operation group did not intervene. After the model was established successfully,the massage group was treated with the method of Shenjin Yisu for 3 weeks,and the sodium hyaluronate group was injected into local joints once a week for 3 weeks,while the model group was not given any treatment. After treatment,the tension of rectus femoris and biceps femoris was tested,and cartilage tissue was stained with HE and then observed under light microscope,and Markin score was made. Analyze the similarities and differences between massage group and other four groups.[Results] The FDD and S values of rectus femoris muscle in the model group were significatly lower than normal group,and those of biceps femoris were significant higher. The FDD and S values in the massage group and sodium hyaluronate group of rectus femoris were significantly higher than the model group (P<0.05),while those of biceps femoris were significantly lower than those of the model group (P<0.05). Comparison with model group,the Mankin's score had statistical significance in massage group and sodium hyaluronate group.[Conclusion] The Shenjin Yigu method can significantly increase the muscle strength,regulate tissue tension and improve the function of joint movement,which is of great significance to protect the function of soft tissue around the knee joint.