基于数据挖掘和网络药理学的中药治疗室性早搏用药规律分析

Analysis of the rule of Chinese medicine in the treatment of ventricular premature beats (VPB) based on data mining and network pharmacology

关键词:  室性早搏  中药  数据挖掘  网络药理  规律分析

Key words:  ventricular premature beats  traditional Chinese medicine  data mining  network pharmacology  law analysis

摘要:

[目的] 探究中药复方治疗室性早搏（VPB）的用药特点及配伍规律。[方法] 以中国知网为数据来源，以主题关键词为“室性早搏＋中医＋临床”、全文关键词为“中药”并且篇名中不含“进展”进行检索。时间选择为1979年1月1日—2020年3月1日。检索出相关中药组方，将组方涉及到的所有药味录入Excel 2010建立数据库，并采用SPSSStatistics 20、SPSS Clementine12统计软件对数据进行分析。使用BATMAN-TCM系统对高频药物所含化合物进行检索，获取这些化合物对应靶点，与Genecard数据库中检索VPB相关靶点相映射并取交集，通过DAVID 6.8数据库进行KEGG通路分析获取关键药物治疗VPB的重要通路，通过Cytoscape 3.2.1软件构建“成分-靶点-关键通路”可视化网络分析。[结果] 纳入的102首处方共含有110味中药，累积频率965次，前20味中药，累计频次562次，占总频率的53.2%。使用频次最高的前3位分别是炙甘草（49次，48.04%）、酸枣仁（43次，42.16%）、丹参（42次，41.18%）。关联规则分析得到6个支持度较高的中药组合，因子分析产生6个公因子。通过网络药理学方法发现酸枣仁活性成分21个，治疗VPB的核心靶点59个，和VPB关系密切的通路11条。[结论] 通过数据分析，发现中医对于VPB的临床治疗以补虚药、安神药、清热药较为常用，多选择药性偏温、平、微寒，药味偏甘，归心、肝、脾经的中药。临床用药时可着重参考酸枣仁、当归、甘松、黄芪、炙甘草、桂枝、生地黄、生龙骨、生牡蛎、麦冬、五味子、川芎、白芍、人参等中药进行配伍使用，初步验证和预测了关键药物酸枣仁治疗VPB的核心靶点和重要通路，为深入研究其作用机制提供参考。

Abstract:

[Objective] To explore the characteristics and compatibility of Chinese herbal compound in the treatment of ventricular premature beats (VPB).[Methods] With CNKI as the data source,the subject keywords were"ventricular extrasystole+traditional Chinese medicine+clinical ",the full text keywords were" traditional Chinese medicine",and the title did not contain "progress" .The time is from January 1,1979 to March 1,2020.Retrieve the relevant Chinese herbal medicine prescriptions,input all the herbs involved into excel 2010 to establish the database,and use SPSS statistics 20,SPSS Clementine12 statistical software to analyze the data.Using the BATMAN-TCM system to retrieve the compounds contained in high-frequency drugs,obtain the corresponding targets of these compounds,map and intersect with the VPB related targets in the Genecard database,analyze the KEGG pathway through the DAVID 6.8 database to obtain the important pathway of key drugs for the treatment of obesity,and use the Cycloscape 3.2.1.The software constructs "component target critical path" visual network analysis.[Results] The 102 prescriptions included in this study contained 110 Chinese herbs,with a cumulative frequency of 965 times,and the first 20 Chinese herbs,with a cumulative frequency of 562 times,accounting for 53.2% of the total frequency.The top three most frequently used were Glycyrrhizae (49 times,48.04%),Semen Ziziphi spinosae (43 times,42.16%) and Salvia miltiorrhiza Bunge (42 times,41.18%).Association rule analysis was used to get 6 Chinese medicine combinations with high support,and factor analysis produced 6 common factors.21 active components of Semen Ziziphi spinosae were found,59 core targets and 11 pathways related to VPB were found by network pharmacology.[Conclusion] Through data analysis,it is found that traditional Chinese medicine is more commonly used in the clinical treatment of VPB,such as tonic drugs,tranquilizing drugs and heat clearing drugs,and most of them are traditional Chinese medicine which has the properties of warming,calming,mild cold,sweet taste,heart returning,liver and spleen channels.In clinical use,we can mainly refer to Semen Ziziphi spinosae,Angelica sinensis,Nardostachys chinensis Batal,Astragalus membranaceus,Glycyrrhizae,Ramulus Cinnamomi,Rehmannia glutinosa Libosch,OsDraconis,Concha Ostreae,Ophiopogon japonicus,Schisandra chinensis,Ligusticum chuanxiong Hort,Radix Paeoniae Alba,Panax ginseng and other traditional Chinese medicine for compatibility.We preliminarily verified and predicted the core target and important pathway of Semen Ziziphi spinosae for VPB treatment,and provided reference for in-depth study of its mechanism.