美洲大蠊粪便提取物对大鼠离体心脏功能的影响研究

Study on the cardiac protection of fecal extracts from Periplaneta americana L. in ex vivo rat hearts

关键词:  美洲大蠊  粪便提取物  离体心脏  正性肌力作用

Key words:  Periplaneta americana L.  fecal extract  ex vivo rat hearts  positive inotropic effect

摘要:

[目的] 探究美洲大蠊粪便提取物（PAF）对大鼠离体心脏功能的影响。[方法] 采用Langendorff灌流装置构建SD大鼠离体心脏灌流模型，检测各组左室收缩压（LVSP）、左心室压力最大上升和下降速率（±dp/dtmax）、心率（HR）等血流动力学指标的变化。[结果] PAF可显著提升正常大鼠离体心脏的LVSP和±dp/dtmax，且呈剂量依赖性，其中0.5 mg/mL的高剂量作用效果最为显著，但PAF对HR并未表现出明显的影响。[结论] PAF升高LVSP，增大±dp/dtmax，对大鼠离体心脏有正性肌力作用，能够增强心肌收缩力。PAF对心率未表现出明显影响，有利于降低心脏耗氧量，可能是其能够发挥心脏保护作用的重要原因。

Abstract:

[Objective] To investigate the cardiac protection of fecal extracts from Periplaneta americana L.(PAF) in ex vivo rat hearts. [Methods] The Langendorff perfusion model of ex vivo rat heart was established for monitoring the changes of the hemodynamic indexes caused by PAF administration, including the left ventricular systolic pressure(LVSP), left ventricular pressure maximum upstroke and decrease velocity (±dp/dtmax), and heart rate (HR). [Results] PAF could significantly enhance LVSP and ±dp/dtmax in ex vivo rat hearts, and was dose-dependent, of which 0.5 mg/mL had the most significant effect, but PAF had not shown a significant effect on HR. [Conclusion] In this study, PAF increased LVSP and ±dp/dtmax, which had a positive inotropic effect in ex vivo rat hearts and could enhance myocardial contractility. In addition, it showed no effect on heart rate, which was beneficial to reduce cardiac oxygen consumption and exert cardioprotection.