“团泊一号”中药样品供试品溶液制备的自动化设备开发与应用

Development and application of "Tuanbo 1" automated equipment for sample solution preparation of traditional Chinese medicine samples

关键词:  供试品溶液制备  自动化设备  中药  高通量

Key words:  preparation of sample solution  automated equipment  traditional Chinese medicine  high throughput

摘要:

目的 研究旨在开发一种用于中药样品制备的自动化设备，以实现高通量、高效率和高质量的样品制备。方法 通过整合自动化技术与传统的中药样品制备方法，研制“团泊一号”中药样品供试品溶液制备的自动化设备。以栀子为实验样本，利用高效液相色谱法分析其京尼平苷的含量，以验证设备的适用性和稳定性，并将其应用于杜仲叶、玄参和牛膝等其他中药样品供试品溶液的制备。通过建立“蛛网”模式的多维度评价系统，综合评估设备性能。结果 “团泊一号”自动化设备成功集成了加液、提取、离心、分离、转移等多个环节，显著提升了中药样品供试品溶液制备的自动化水平。在栀子样品制备中，该设备展现出良好的适用性和稳定性。相较于传统的人工制备供试品溶液，该设备在处理效率、自动化程度、准确性、一体化程度和成本效益等方面均显示出明显优势。结论 “团泊一号”自动化设备能够有效替代传统的人工操作，适用于多种中药样品供试品溶液的制备。该设备能显著提升样品处理量与效率，同时降低成本，具有推广应用的潜力。

Abstract:

Objective This study aims to develop an automated equipment for the preparation of traditional Chinese medicine(TCM) samples, enhancing throughput, efficiency, and quality in sample preparation.Methods By integrating automation technology with traditional sample preparation methods, we developed "Tuanbo 1" for the automated preparation of TCM sample solutions. Taking Fructus Gardeniae as an example, the content of geniposide was analyzed using high performance liquid chromatography to verify the applicability and stability of the equipment. In addition, the equipment was employed to prepare sample solutions of other TCMs, such as Folium Eucommiae, Radix Scrophulariae, and Radix Achyranthis Bidentatae. The performance of the equipment was comprehensively assessed by establishing a "spider-web" model of a multi-dimensional evaluation system.Results The "Tuanbo 1" automated equipment successfully integrated multiple steps including liquid addition, extraction, centrifugation, separation, and transfer, greatly enhancing the automatic level of TCM sample preparation. In the preparation of Fructus Gardeniae, the equipment demonstrated strong applicability and stability. In comparison to traditional manual sample preparation, the equipment exhibited significant advantages in terms of processing efficiency, automation level, accuracy, integration, and cost-effectiveness.Conclusion The "Tuanbo 1" automated equipment can effectively replace traditional manual operations and is suitable for the preparation of various TCM sample solutions. It offers considerable improvements in sample processing capacity and efficiency while reducing costs, indicating potential for widespread application.