丹酚酸B诱导体外培养大鼠骨髓基质细胞向心肌样细胞分化的研究*

The Experimental Study of Isolation and Culturating of Bone Marrow Stromal Cell and Differentiation into Cardiomyocyto-Like Cells Induced by Salviandic Acid B in Vitro

关键词:  丹酚酸B  骨髓基质细胞  诱导  心肌样细胞

Key words:  salvianolic acid B  marrow stromal cells  induction  cardiac-like muscle cells

摘要:

[目的]探讨大鼠骨髓基质细胞的体外培养及丹酚酸B(Salvianolic acid B)诱导其向心肌样细胞分化的可能。[方法]取大鼠股骨及胫骨干骨髓基质细胞(MSCs)培养,保留贴壁细胞进行传代;免疫细胞化学行肌动蛋白抗原、肌钙蛋白T抗原染色;观察丹酚酸B对骨髓基质细胞向心肌细胞分化的影响。[结果]大鼠骨髓基质细胞在形态上呈贴壁集落生长,具有成纤维细胞样外观。丹酚酸B加入5-氮胞苷诱导组后细胞死亡较少,长方形、多角形细胞明显增多。[结论]丹酚酸B能够促进体外诱导骨髓基质细胞向心肌样细胞的分化。

Abstract:

[Objective] To discuss the growth and spread of rat marrow stromal cells (rMSCS) in vitro and the probability of Salvianolic acid B induce rMSCS to cardiac-like muscle cells.[Methods] The rMSCS from adult rats,which attached to the walls in cell culture,were isolated and purified for study.The cardiomyogenic induction was started by adding Salvianolic acid B to the culture medium,and the proliferation and differentiation of them were observed.[Results] The rMSCS grew in colonies,toke the appearance of fibroblasts and differentiated into cardiac-like muscle cells after adding Salvianolic acid B. The rate of differentiation approximated 30% in 4 weeks.[Conclusion] Cardiomyocytes can be generated from rMSCS in vitro,and the marrow stromal cell is one of the best resources of donor cardiomvocvtes.