“益气调血, 扶本培元”针法对多发梗死性痴呆模型大鼠联合型学习能力的影响

Effects of "Yiqitiaoxue, fubenpeiyuan" Acupunctur Method on Associative Learning in Rat of Multi-infarct Dementia

关键词:  血管性痴呆  穿梭箱实验  学习记忆  针灸疗法

Key words:  vascular dementia  shuttle box test  learning and memory  acup thet

摘要:

[目的]探讨多发梗死性痴呆(MID)模型大鼠的行为学特征及“益气调血,扶本培元”针法的疗效。[方法]采用栓子注入法制作多发梗死性痴呆模型,分为模型组、针刺组和非穴组,并设正常组和假手术组。应用穿梭箱实验观测各组大鼠联合型学习能力的变化,并分析针刺对大鼠学习能力的影响。[结果]与对照组比较,模型组大鼠学习记忆功能明显受损,其被动回避潜伏期明显延长,主动回避反应率明显降低。针刺后,针刺组被动回避潜伏期与非穴组比较明显缩短(P<0.05),其主动回避反应率较模型组及非穴组明显增高(P<0.05),而与正常组及假手术组比较,已无统计学意义上的差异。非穴组与模型组比较无显著性差异。[结论]“益气调血,扶本培元”针法可改善多发梗死性痴呆模型大鼠的联合型学习能力,且具有腧穴特异性。

Abstract:

[Objective] To explore the change of associative learning in rat of multi-infarct dementia (MID) and the influence of "Yiqitiaoxue,fuhenpeiyuan" acupuncture method on it by ob servation of th behavioral features per group. [Methods] The multiinfarct dementia model was made by injecting emboli into the internal carotid artery. Of them 43 proved dementia by shuttle box weallocated to 3 groups: model,acup and sham-acupuncture, meanwhile 15 normal and sham operated rats were used as control respectively. Shuttle box test were employed to assess associative learning ability per group and to analyse the curative effect of acup [Results] Companed to normal and sham operation group, Model animals showed significant learning and memory impairments: longer passive avoidance latency (PAL) and lower active avoidance response rate (AARR) in shuttle box test (P<0.05);Interestingly, the animals exposed to acupuncture have remarked shorter PAL and higher AARR, whereas, no difference between sham-acupuncture and model group. [Conelusion] These data suggest that "Yiqitiaoxue,fubenpeiyuan" acupuncture method can improve associative learning in rats of MID and points have specificity of treatment.