HPLC法测定降香挥发油羟丙基-β-环糊精包合物中反式-橙花叔醇

Determination of Trans-nerolidol in Inclusion Complex of Volatile Oil from Lignum Dalbergiae Odoriferae with Hydroxypropyl-β-Cyclodextrin by HPLC Method

关键词:  羟丙基-β-环糊精包合物  降香挥发油  反式-橙花叔醇  高效液相色谱法

Key words:  hydroxypropyl-β-cyclodextrin inclusion complex  volatile oil  Lignum dalbergiae odoriferae  trans-nerolidol  HPLC method

摘要:

[目的]建立用H PLC法测定降香挥发油羟丙基-β-环糊精包合物中反式-橙花叔醇的方法。[方法]色谱柱:Diamonsil C₁₈(250×4.6mm,5μ);流动相:甲醇-水(85:15);流速:1.0mL/min;检测波长:210nm;柱温30℃。[结果]反式-橙花叔醇线性范围为0.1×10^-5～0.6×10^-3μL,r=0.9999,加样回收率为102.62%,RSD为1.314%(n=9)。[结论]本方法简便、快速,精密度、重现性好,可以作为降香挥发油羟丙基-β-环糊精包合物的质量控制方法。

Abstract:

[Objective] To establish a HPLC method for determination of trans-nerolidol in the Inclusion Complex of the volatile oil from Lignum dalbergiae odoriferae with Hydrox-ypropyl-β-Cyclodextrin. [Methods] The HPLC separation was performed on a Diamonsil C,A column (250×4.6 mm, 5 μ) with(olumn temperature at 30℃,a mobile phase consisted of methanol-water (85:15) with a flow rate of 1.0 mL/min and the UV detector wavelength was 210 nm. [Result] The calibration curve was linear in the concentration range of 0.1×10^-5~0.6×10^-3 μL (r=0.999 9). The mean recovery rate was 102.62% and the RSD was 1.314% (n=9). [Conclusion] This method was simple, rapid, accurate,reproducible and can be used for the quality control of the Inclusion Complex of the volatile oil from Lignum dalbergiae odoriferae with Hydroxypropyl-β-Cyclodextrin.