摩腹法对肠易激综合征模型结肠组织脑肠肽表达的影响*

Influence of Rubbing abdomen on express of braingut petide in colon tissue in Iirritable Bowel Syndrome model

关键词:  肠易激综合征  P物质  血管活性肠肽  摩腹法

Key words:  iirritable bowel syndrome  SPa VIP  rubbing abdomen

摘要:

[目的]从脑肠肽角度探讨摩腹法干预肠易激综合征(IBS)的作用机制。[方法]把新西兰白兔随机分为正常组、模型组和手法组,模型组和手法组用0～2℃冰水灌胃21d以建立肠易激综合征,手法组运用摩腹法操作10d,采用免疫组化方法对以上3组结肠组织P物质(SP)、血管活性肠肽(VIP)、胆囊收缩素(CCK)进行半定量分析。[结果]模型组结肠黏膜SP表达的阳性面积、不透光率密度值明显减少,VIP增多(P<0.05),经摩腹干预后,SP表达升高,VIP降低,与正常组比较无显著性差异(P>0.05),3组CCK表达无显著性差异(P>0.05)。[结论]结肠SP和VIP表达异常可能是IBS的致病因素之一,摩腹法可以通过调节局部肠道SP和VIP的功能来治疗IBS。

Abstract:

[Objection] To investigate the mechanism Rubbing abdomen on treating Iirritable Bowel Syndrome in point of view braingut petide.[Methods] The New Zealand male albino rabbits were randomly divided into normal group, model group, Rubbing abdomen group. The rabbits were lavaged with 0～2 ℃ icy water in model group and Rubbing abdomen group for 21 days, Then they were applied to Rubbing abdomen in the third group for 10 days. The above each group were analyzed semiquantitatively on SP、VIP、CCK of colon tissue with the immunohistochemistry method.However, VIP were increased(P<0.05).[Results] The immunoreactive area and Opacity density of SP in colon tissue were obviously impairment.After the treatment of Rubbing abdomen,the express of SP was elevated, VIP depressd.[Conclusion] The abnormal express of SP and CCK were probable one of etiological factor of IBS.The manipulation of Rubbing abdomen may be regulate the function of SP and CCK in colon tissue of IBS.