彩色多普勒超声测量大鼠心功能的可行性研究

Feasibility of color doppler ultrasound in assessment of heart function in rat

关键词:  彩色多普勒超声  心肌梗死  大鼠心脏功能  血流动力学

Key words:  color Doppler ultrasound  myocardial infarction  cardiac function of rat  hemodynamics

摘要:

[目的]通过与血流动力学检测比较确定彩色多普勒超声是否可用来评价正常和慢性心力衰竭大鼠左心室结构和功能变化。[方法]利用冠状动脉左前降支结扎术制备大鼠慢性心力衰竭模型,手术后5～7周采用无创彩色多普勒超声和有创血流动力学测定大鼠心功能,同时以正常大鼠进行平行对照。[结果]用彩色多普勒超声测定心脏功能正常大鼠(NOR组)和慢性充血性心力衰竭大鼠(CHF组)的射血分数(EF)和短轴缩短率(FS)值与用血流动力学方法测得的左室压力变化最大速率(LV+dp/dt_max)值呈良好的正相关(r=0.921和r=0.827,均P<0.01)。[结论]无创性经胸壁二维引导彩色多普勒超声可用来代替有创血流动力学评价慢性心力衰竭大鼠心脏结构和功能改变。

Abstract:

[Objection] This study attempted to determine whether color Doppler ultrasound examination was available to evaluate the change of left ventricle structure and function in normal rats or rats with chronic heart failure through a comparison with the hemodynamic detection.[Methods] By ligating the left anterior descending coronary artery the chronic heart failure was induced in rats. Five to seven weeks after operation the heart function was measured with color Doppler ultrasound and invasive hemodynamic determination. Simultaneously, the parallel control experiment was performed.[Results] A perfect correlation was found between the value of EF and FS obtained with color ultrasound and LV peak +dp/dt_max determined with hymodynamic research in both normal and CHF rats(r=0.921 and r=0.827, respectively, P<0.01).[Conclusion] The noninvasive twodimensional color Doppler ultrasound at chest wall can assess the change of heart function and structure in rats with chronic cardiac failure substituting for the invasive hymodynamic evalustion.