丹酚酸B干预骨髓间充质干细胞移植对AMI大鼠心梗区血管新生的*

Angiogenic effect of bone marrow mesenchyma stem cells transplantation intervened by SalB on acute myocardial infarcts in rats infarction in rats

关键词:  丹酚酸B  骨髓间充质干细胞  心肌缺血  细胞移植  血管新生

Key words:  Salvianolic acid B  MSCs  ischemic heart disease  cell therapy  angiogenesis

摘要:

[目的]探讨丹参单体丹酚酸B(SalB)干预骨髓间充质干细胞(MSCs)心肌移植及其对大鼠心梗区毛细血管新生的影响。[方法]选用成年近交系Wistar大鼠建立心肌缺血模型。将造模成功的75只大鼠随机分为5组。冠脉结扎术后,联合组给于大鼠SalB并移植MSCs(SalB+B组),细胞移植组(B组)移植等量的MSC,中药组给予等量的SalB(SalB组),对照组注射等容积的MSCs完全培养液(S组),模型组(C组)。手术后2周,通过心肌组织CD34免疫组化染色,观察丹酚酸B干预MSCs心肌移植对血管新生的影响。[结果]CD34染色示联合组动物心肌梗死区毛细血管密度高于B组和SalB组,且有少数细胞形成血管样结构。[结论]在急性心肌梗死(AMI)大鼠心肌移植MSCs,通过参与血管形成,改善梗死区的血液供应,是SalB起效的重要物质基础之一,具有良好的开发和应用前景。

Abstract:

[Objective] To evaluate the angiogenic effect of the bone marrow mesenchymal stem cells (MSCs) transfected with SalB on myocardial infarcts in rats[Methods] The animal model of heart ischemic was established by ligating the left anterior descending coronary artery in Wistar rats.The ligated rats were divided into 5 groups (n=15each),and they were injected MSC at the heart infarct zone with SalB (Group SalB+B),MSCs (Group B),SalB (Group SalB),sham operation (Group S) and model.Two weeks after the injection,the capillary density of the infracted zone and the expression of CD34 in vivo were examined.[Results] Two weeks after the transplantation,the capillary density was significantly greater in Group SalB+B than that in Group B and Group SalB.[Conclusion] Bone marrow mesenchyma stem cells interfered with SalB on myocardial infarcts in rats could provide the blood of infarction zone and maybe form new vasculars to repair infracted myocardium,and SalB is one of the important material basal in Danshen and have satisfactory development and application prospect.