| 摘要: |
| [目的]建立陕西产延胡索药材的高效液相色谱(HPLC)指纹图谱,为延胡索药材制定质量标准提供参考。[方法]采用反相高效液相色谱法,KromasilC18色谱柱,柱温30℃,流动相:乙腈-醋酸盐缓冲液(pH6.0)梯度洗脱,流速1.0mL/min,检测波长280nm,洗脱时间75min。[结果]建立了以延胡索乙素为内参照峰的延胡索药材HPLC指纹图谱,共含有17个共有色谱峰。[结论]建立的指纹图谱可以作为延胡索质量评价和品种鉴别的主要依据之一。 |
| 关键词: 延胡索 HPLC指纹图谱 延胡索乙素 |
| DOI：10.11656/j.issn.1673-9043.2007.03.17 |
| 分类号: |
| 基金项目:*国家自然基金面上项目(30672664);天津市高等学校科技发展基金项目(20040309) |
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| Study of fingerprint of rhizoma corydalis by HPLC |
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DOU Zhi-ying, SUN Wei, ZHANG Min
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TianJin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi.The conclusion makes reference to standard of quality control of Rhizoma Corydalis.[Methods] The methods were performed by RP-HPLC.Kromasil ODS-C18 Column was used at 30℃,acetonitrile-acetate buffer solution (pH 6.0) as mobile phase,gradient eluting,flow rate was 1.0 mL/min,detection wavelength was set at 280 nm and eluting period were 75 min.[Results] HPLC-fingerprint of Rhizoma Corydalis was established,which contained 17 common peaks.[Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis. |
| Key words: Rhizoma Corydalis HPLC-fingerprint Tetrahydropalmatine |
