终末糖基化产物、高脂诱导内皮细胞凋亡的作用途径及葛根素的保护作用*

Advanced glycosylation end products and high fat-induced apoptosis in human umbilical vein endothelial cells and the protective effect of puerarin

关键词:  内皮细胞  凋亡  葛根素

Key words:  endothelial cells  apoptosis  puerarin

摘要:

[目的]探讨葡萄糖和高脂诱导人脐静脉内皮细胞凋亡的作用途径及葛根素的保护作用。[方法]采用终末糖基化产物、高脂共同诱导内皮细胞凋亡建立模型,并用葛根素进行干预。采用免疫细胞化学法检测Cas-pase3阳性细胞数。流式细胞分析仪检测终末糖基化产物及高脂预处理后内皮细胞凋亡百分率。[结果]中浓度终末糖基化产物(AGE50μg/mo)l以及氧化低密度脂蛋白(ox-LDL50mg/L)36h作用于人脐静脉内皮细胞与正常水平的内皮细胞相比,凋亡细胞数量显著增加P<0.05,葛根素预处理12h后显著降低高糖诱导的凋亡率P<0.05。[结论]葛根素可以抑制高糖高脂诱导的人脐静脉内皮细胞凋亡,这在防治糖尿病及动脉粥样硬化并发症的过程中起到一定作用。

Abstract:

[Objective] Hyperglycemia-induced apoptosis in vascular endothelial cells may be contributed to the acceleration of atherosclerosis associated with diabetes. This study confirmed that acylated ghrelin attenuated hyperglycemia-induced apoptosis in cultured human umbilical vein endothelial cells (ECV-304).[Methods] Advanced glycosylation end products and high fat were used to establish the cellular model, Caspase3 protein expression was detected by immunocytochemistry. The apoptotic cells were determined by flow cytometric analysis.[Results] Compared with normal glucose, exposure of ECV-304 in hyperglycemia level for 36 h significantly increased the number of apoptotic cells, but pretreatment with puerarin for 12 h and then culturing for 36 h could attenuate hyperglycemia-induced apoptosis significantly (P<0.05).[Conclusion] The results of our study indicated that puerarin could inhibit high glucose and fat induced apoptosis in human umbilical vein endothelial cell, having some effect in prevention against the complications of diabetes and atherosclerosis.