开郁清热方对自发2型糖尿病大鼠胰岛β细胞功能的影响*

Effect of Kaiyu Qingre Prescription on function of β-cell of rats with spontaneous type 2 of diabetes

关键词:  2型糖尿病  糖代谢  开郁清热  β细胞功能

Key words:  type 2 diabetes mellitus  glucose metabolism  Kaiyuqingre  β-cell function

摘要:

[目的]观察开郁清热中药对自发2型糖尿病大鼠胰岛β细胞功能的影响。[方法]引进自发性2型糖尿病大鼠(OLETF)及正常对照组同系非糖尿病大鼠(LETO),将已发病OLETF大鼠分为模型组、罗格列酮组、开郁清热组,灌胃12周,检测其口服糖耐量、胰岛素分泌、胰岛素分泌指数、免疫组化法检测胰岛细胞胰岛素表达。[结果]开郁清热组口服葡萄糖曲线下面积较模型组下调,胰岛素曲线下面积较模型组上调,胰岛素分泌指数增加,免疫组化胰岛素染色阳性面积增加,积分下吸光度上调。[结论]开郁清热方对自发2型糖尿病大鼠糖代谢有较好干预作用,其机制与改善胰岛β细胞功能有关。

Abstract:

[Objective] To observe the effects of Kaiyuqingre (dissipating depression of QI and clearing away heat,KYQR) on preserving β-cell function in rats with spontaneous type 2 diabetes (Otsuka Long Evans Tokushima Fatty rats,OLETF).[Methods] The OLETF rats were administrated with KYQR for twelve weeks,using rosiglitazone as the control.At the end,oral glucose tolerance test were carried out to evaluate islets function,and the blood sugar,insulin secretion,insulin secretion index were measured with imohistochemistry stain and computer image analysis technique was used to determine the expression of insulin in islets.[Results] KYQR had less glucose area under the curve and more insulin area under the curve in oral glucose tolerance test than the model group.The KYQR group insulin secretion index was improved.Immunohistochemistry stain of islets demonstrated that KYQR could improve the expression of insulin.[Conclusion] KYQR can effectively regulate glucose metabolism of rats with type 2 diabetes.and its mechanism may involve preserving the β-cell function.