HPLC法测定附子配伍前后乌头类生物碱含量

Determination of Aconitine alkaloids before and after compatibility of Radix Aconiti Lateralis preparata with HPLC

关键词:  附子  干姜  生物碱  含量测定

Key words:  Radix Aconiti Lateralis  Radix Ginger  alkaloid  quantitative analysis

摘要:

[目的]用高效液相色谱(HPLC)法同时测定附子、附姜、四逆汤水提液中新乌头碱、乌头碱、次乌头碱的含量,研究配伍前后水提液化学成分变化,以验证中药配伍理论。[方法]Waterssymmetry C₁₈色谱柱(4.6mm×150mm,5μm),流动相为甲醇-0.1%三乙胺(60:40);检测波长:235nm,柱温:35℃;流速:1.0mL/min。[结果]附子配伍后次乌头碱增加显著,推测可能为水解产物的X峰明显增大。其中附姜配伍3种生物碱均增加;而四逆汤中新乌头碱略有增加,乌头碱明显降低。[结论]该方法准确、简便,重复性好,可说明附子配伍前后乌头类生物碱变化规律。

Abstract:

[Objective] HPLC was used to simultaneous determining the content of Mesaconitine,Aconitine and Hypaconitine in the water extract of Radix Aconiti Lateralis preparata,Radix Aconiti Lateralis preparata and Radix Ginger,Sini Decoction.The changes of the chemical composition in the water extract were studied to verify the compatibility theory of Chinese medicine.[Methods] Determination was performed by HPLC method,a waters Symmetry C₁₈ (150 mm×4.6 mm.5 μm) was adopted with a mobile phase of methanol -0.1% triethylamine(60:40) solution at a flow rate of 1.0 mL/min;The detection wavelength was set at 235 nm and the column temperature was 35℃.[Results] The content of Hypaconitine was greatly increased after Radix Aconiti Lateralis preparata compatibility,suggesting that obvious increasing of X composition in the hydrolysate.Among them,the content of mesaconitine,aconitine was increased in Radix AeonitiLateralis preparata water extract with Radix Ginger.The content of mesaconitine was increased minimally and aconitine was greatly decreased in the sini Decoction.[Conclusion] The method was simple,precise and reliable for determination of aconitine alkaloids.It can illuminated the change rules of aconitine alkaloids before and after Radix Aconiti Lateralis preparata compatibility.