心脑血脉宁减少家兔易损斑块模型斑块内血肿、稳定斑块的研究*

Effect of Xinnao Xuemaining on reducing plaque hematoma and stable plaques of vulnerable plaque in rabbits with atherosclerosis

关键词:  心脑血脉宁  易损斑块  新生血管  血小板源性生长因子受体  周细胞

Key words:  Xinnao Xuemaining  vulnerable plaque  neovascularization  platelet derived growth factor receptor  pericytes

摘要:

[目的]探讨心脑血脉宁对家兔动脉粥样硬化易损斑块模型斑块内血肿、斑块内新生血管成熟的影响。[方法]通过喂养高脂饲料和主动脉球囊内皮拉伤术复制动脉粥样硬化易损斑块模型,实验分为模型组,假手术组,心脑血脉宁组,历时15周。处死后,取腹主动脉行苏木精-伊红(HE)染色,观察新生血管结构,计数斑块出血数,免疫组化方法检测血小板源性生长因子受体的表达。[结果]1)HE观察:模型组有6只家兔的12斑块发生血肿,而心脑血脉宁组有3只家兔的3斑块发生血肿。模型组新生血管周围缺乏周细胞包绕,而心脑血脉宁干预后,新生血管周围的周细胞明显增多。2)免疫组化结果:心脑血脉宁可上调血小板源性生长因子受体(PDGFR)蛋白增加,与模型组相比差异有显著性,P<0.01。[结论]心脑血脉宁干预家兔动脉粥样硬化易损斑块模型,能减少斑块内血肿,促斑块内新生血管成熟,其机制可能与上调PDGFR的表达,募集周细胞有关。

Abstract:

[Objective] To observe the effects of Xinnao Xuemaining on reducing plaque hematoma and the expression of PDGFR of vulnerable plaque in rabbits with atherosclerosis.[Methods] The vulnerable atherosclerotic plaque model was reproduced by operation on abdominal aorta endothelium for inducing sacculus pull injury with the foraging with high cholesterol lipid feeding.Ribbits were divided into three groups:model group,sham operation group and Xinnao Xuemaining group.The experiment lasted for 15 weeks.After killing at the end of experiment,the sample of abdominal aorta was collected for HE staining and microscopic lesion examination,counting of the number of plaque hematoma and detecting the protein content of PDGFR by immunohistochemistry.[Results] HE observation was found 6 rabbits of the 12 plaque hematoma in model group,and three rabbits,three plaque hematoma in Xinnao Xuemaining group.The new blood vessels lacked pericytes surrounded,and after the intervention of Xinnao Xuemaining,pericytes surrounded the new blood vessels increased.The results of immunohistochemical staining showed that the expression of platelet derived growth factor receptor in Xinnao Xuemaining group enhanced significantly compared with model group (P<0.01).[Conclusion] Xinnao Xuemaining can reduce plaque hematoma of vulnerable plaque in rabbits with atherosclerosis and promote plaque neovascularization mature,whose mechanism may be related to increased expression of PDGFR and raised on pericytes.