| 摘要: |
| [目的]观察银杏叶注射液对体外培养的人脑微血管内皮细胞增殖的影响。[方法]体外培养人脑微血管内皮细胞(HBMEC),采用四甲基偶氮唑蓝(MTT)法测定不同浓度银杏叶注射液对HBMEC增殖的影响。[结果]与对照组相比较,银杏叶注射液浓度为70mg/L时,对血管内皮细胞无促增殖作用;浓度为140、210、280、350mg/L时均具有促进血管内皮细胞增殖的作用,但各组之间相比较无统计学意义。[结论]银杏叶注射液具有促进人脑微血管内皮细胞增殖的作用。 |
| 关键词: 银杏叶注射液 人脑微血管内皮细胞 四甲基偶氮唑盐法 |
| DOI：10.11656/j.issn.1673-9043.2011.01.15 |
| 分类号:R289.5 |
| 基金项目:*国家自然科学基金项目(30873467) |
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| MTT assay measured impact of Ginkgo Biloba Injection on proliferation of microvascular endothelial cell in human brain |
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LI Zhuo,WANG Ping
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| Abstract: |
| [Objective] To explore the impact of ginkgo biloba injection on the proliferation of in vitro-cultured microvascular endothelial cells (HBMEC) in human brain.[Methods] HBMEC was cultured in vitro.MTT assay was applied to detect the impacts of ginkgo biloba injection at different concentrations on HBMEC.[Results] When concentration of ginkgo biloba injection was 70mg/L it could not influence the proliferation;when was 140mg/L, 210mg/L,280mg/L and 350mg/L,all they could promote the proliferation of microvascular endothelial cells significantly,but there was no statistically difference between any two groups.[Conclusion] Ginkgo biloba injection can promote the proliferation of HBMEC. |
| Key words: Ginkgo biloba injection HBMEC MTT assay |
