暑热宁合剂薄层色谱鉴别*

Thin-layer chromatography identification of Shurening mixture by TCL

关键词:  暑热宁合剂  薄层色谱  大青叶  黄连  香薷  半夏

Key words:  Shurening mixture  TCL  Folium Isatidis  Rhizoma Coptidis  Herba Moslae  Cortex Magnoliae Officinalis  Rhizoma Pinelliae

摘要:

[目的]建立暑热宁合剂薄层色谱的鉴别方法,为其质量标准的制定提供依据。[方法]采用薄层色谱法对暑热宁合剂中的大青叶、黄连、香薷、厚朴和半夏进行定性鉴别。[结果]在薄层色谱中,供试品溶液在与对照药材或是对照品相应位置上显相同颜色的斑点,且斑点清晰,阴性对照在相同位置上无对应的斑点。[结论]所建立的薄层色谱定性方法专属性强,可用于暑热宁合剂的质量控制。

Abstract:

[Objective] To establish a thin layer chromatographic (TCL) method for identification of Shurening mixture and provide the basis for the establishment of its quality standard.[Methods] Folium Isatidis,Rhizoma Coptidis,Herba Moslae,Cortex Magnoliae Officinalis and Rhizoma Pinelliae in Shurening mixture were identified by TCL method.[Results] Folium Isatidis,Rhizoma Coptidis,Herba Moslae,Cortex Magnoliae Officinalis and Rhizoma Pinelliae could be identified by TCL in the same site,and the spots were very clear.There were no corresponding spots at the same site in the negative sample.[Conclusion] TCL is a simple,reliable and specific method with good reproducibility,and can be used for the quality control of Shurening mixture.