高效液相色谱法测定生姜中6-姜酚的含量*

Determination of 6-gingerol in ginger by high performance liquid chromatography

关键词:  生姜  6-姜酚  高效液相色谱(HPLC)

Key words:  ginger  6-gingerol  HPLC

摘要:

[目的]建立测定生姜中6-姜酚的高效液相色谱(HPLC)法。[方法]以100倍80%的甲醇热回流提取生姜2 h后用HPLC法测定;采用依利特C₁₈反相色谱柱(250 mm×4.6 mm,5μm);以异丙醇-甲醇-冰乙酸-水(12:38:4:36,V/V/V/V)为流动相;流速0.8 mL/min;紫外检测波长280 nm;柱温34℃。[结果]6-姜酚在0.021 6~0.432 0μg的质量范围内与峰面积线性关系良好(r²=0.999 2,n=6),回收率为(100.79±2.84)%,RSD为2.84%。[结论]本法操作简便,灵敏度高,快速可靠,可用于生姜中6-姜酚含量的测定。

Abstract:

[Objective] To establish a HPLC method for the determination of 6-gingerol in ginger.[Methods] Ginger was extracted by heat reflux with 80% methanol for 2 hours,the drug ratio was 1:100,and the Hypersil C₁₈ ODS (250 mm×4.6 mm,5μm) column was used to determine.The mobile phase consisted of methanol-water-isopropyl-alcohol-glacial acetic acid (38:36:12:4);The flow rate was 0.8 mL/min.The detection wavelength was 280 nm.The column temperature was kept at 34℃.[Results] The linear range was between 0.021 6~0.432 0μg.And the average recovery of the method was (100.79±2.84)% and the RSD was 2.84%.[Conclusion] The results show that this assay method is simple and accurate.And the method can be used to determine the contents of 6-JF in Zingiber of-ficinale Rose.