蒺藜全草中甾体皂苷类化学成分研究

Study on chemical components of Steroidal Saponins from Tribulus terrestris L.

关键词:  蒺藜  甾体皂苷  分离纯化  结构鉴定

Key words:  Tribulus terrestris L.  steroidal saponins  isolation and purification  structural elucidation

摘要:

[目的] 对蒺藜全草化学成分进行研究。[方法] 采用大孔吸附树脂柱色谱、硅胶柱色谱、ODS柱色谱及制备高效液相色谱法(PHPLC)对蒺藜70%乙醇提取液进行分离纯化,并利用理化数据测定和光谱数据分析等方法对所得单体进行了结构鉴定。[结果] 分离鉴定了5个甾体皂苷:1)uttroside B;2)polianthoside D;3)替告皂苷元-3-O-β-D-吡喃木糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-吡喃葡糖基(1→4)-[α-L-鼠李糖基(1→2)]-β-D-吡喃半乳糖苷;4)(25R)-26-O-β-D-吡喃葡糖基-5α-呋甾-3β,22α,26-三醇-3-O-β-D-吡喃葡萄糖基(1→4)-吡喃半乳糖苷;5)海柯皂苷元-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷。[结论] 化合物1和2为首次从蒺藜中分离获得。

Abstract:

[Objective] To investigate the chemical components of the herb Tribulus terrestris L. [Methods] The chemical components were isolated through column chromatography on macroporous resin (SP825), silica gel, octadecyl silica (ODS) columns and PHPLC. Their structures were identified by physicochemical properties and spectra data. [Results] Five steroidal saponins were obtained from Tribulus terrestris L. They were uttroside B (1), polianthoside (2), tigogenin-3-O-β-D-xylopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside (3), (25R)-26-O-β-D-glucopyranosyl-5α-furost-12-one-3β, 22α, 26-triol-3-O-β-D-gluco-pyranosyl-(1→4)-β-D-galactopyranoside (4) and hecogenin-3-O-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (5). [Conclusion] Compounds 1 and 2 were obtained from Tribulus terrestris L. for the first time.